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Cloning and Expression of the MPD Gene from Arthrobacter Ureafaciens Strain and Analysis by Bioinformatics

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成果类型:
会议论文
作者:
Li, Dandan*;Yuan, Yongze(袁永泽);Yu, Jinhui;Yu, Wen;Li, Jinglong;...
通讯作者:
Li, Dandan
作者机构:
[Yuan, Yongze; Yu, Jinhui; Xiong, Li; Liu, Deli; Li, Jinglong; Yu, Wen; Li, Dandan] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构:
[Li, Dandan] C
Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
语种:
英文
期刊:
2014 INTERNATIONAL CONFERENCE ON BIOLOGICAL ENGINEERING AND BIOMEDICAL (BEAB 2014)
年:
2014
页码:
47-52
会议名称:
International Conference on Biological Engineering and Biomedical (BEAB)
会议时间:
JAN 10-12, 2014
会议地点:
Yichang, PEOPLES R CHINA
会议主办单位:
[Li, Dandan;Yuan, Yongze;Yu, Jinhui;Yu, Wen;Li, Jinglong;Xiong, Li;Liu, Deli] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
会议赞助商:
Shanghai Jiaotong Univ, Nankai Univ
出版地:
439 DUKE STREET, LANCASTER, PA 17602-4967 USA
出版者:
DESTECH PUBLICATIONS, INC
ISBN:
978-1-60595-162-1
机构署名:
本校为第一且通讯机构
院系归属:
生命科学学院
摘要:
In this study, a bacterium strain, CZ-31, capable of degrading methyl parathion (MP) was isolated from active sludge of a wastewater treatment pond of a pesticide factory in Hubei province. According to morphological characteristics, physiological properties, and 16S rDNA sequence analysis, the isolates CZ-31 were identified as Arthrobacter ureafaciens. Methyl parathion degradation enzyme mpd gene was cloned by PCR from the genome of strain CZ-31. Nucleotide sequencing indicated that the mpd gene contains 996bp and is predicated to encode a peptide of 331 amino acids. The recombinant expressio...

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