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Mitochondrial response and calcium ion change in apoptotic insect cells induced by SfaMNPV

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成果类型:
期刊论文
作者:
Xiu, MH;Peng, JX*;Hong, HZ
通讯作者:
Peng, JX
作者机构:
[Xiu, MH; Peng, JX; Hong, HZ] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Inst Entomol, Wuhan 430079, Peoples R China.
通讯机构:
[Peng, JX] C
Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Inst Entomol, Wuhan 430079, Peoples R China.
语种:
英文
关键词:
baculovirus;Spodoptera litura cell line (SL-1);apoptosis;mitochondria;Ca2+;cytochrome c
关键词(中文):
钙离子;细胞排列;线粒体;细胞色素
期刊:
科学通报(英文版)
ISSN:
2095-9273
年:
2005
卷:
50
期:
12
页码:
1191-1198
基金类别:
Acknowledgements We thank Dr. Max Bergoin for a critical review of the manuscript. This work was supported by the National Natural Science Foundation of China (Grant Nos. 30370056 & 30470073).
机构署名:
本校为第一且通讯机构
院系归属:
生命科学学院
化学学院
摘要:
Mitochondrial responses and changes of calcium ions in apoptotic insect SL-1 cells induced by Syngrapha falcifera multiple nuclear polyhedrosis virus (SfaMNPV) are reported in this paper. By using Rhodamine 123 as a fluorescent labeling probe, flow cytometry analysis and confocal laser scanning microscope observation we observed that the mitochondrial transmembrane potential (ΔΨm) began to decrease in SL-1 cells at 4 h post infection and Δψm reduced continuously with the extension of virus infection. Western blotting indicated that the Bcl-...

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