Biomedical detection needs to develop more detection methods and reagents. CuFeS2, an I-III-IV ternary semiconductor material, has attracted great interest of researchers because of its optical, electrical, low toxicity and controllable chemical synthesis. Here, we synthesized CuFeS2 quantumdots (QDs) with hot-injectionmethod and their ECL behaviors were investigated for the first time, involving the co-reactant anode/cathode ECL emission and the related ECL mechanism. Moreover, a novel ECL immunosensor based on CuFeS2 QDs for the ultrasensitive detection of Cyclin D1 (CCND1) was designed and ...