期刊论文

中文

微生物学杂志

1005-7021

2017

37

1

64-69

10.3969/j.issn.1005-7021.2017.01.010

国家自然科学基金项目（31371776）；

本校为其他机构

单核细胞增生性李斯特菌(Listeria monocytogenes,LM)是人畜共患的食源性致病菌,其对恶劣环境有较强的抵抗力,广泛存在于各种食品加工环境,容易引起严重的食品安全问题。LM是一种胞内寄生菌,其毒力因子内化素A(internalin A,InlA)和内化素B(internalin B,InlB)被认为在LM穿透宿主屏障、入侵宿主细胞以及胞间传播等过程中起到重要作用。本文利用同源重组法将LM野生菌株EGDe的inlA和inlB基因的敲除,对inlA和inlB基因缺失菌株的基本生物学特性进行研究,并运用RealTime-PCR监测LM的毒力基因表达。实验结果表明,基因的缺失对突变菌株的生长以及对环境中的氯化钠(NaCl)和乙醇(EtOH)的耐受能力没有影响...

Listeria monocytogenes（ LM） is a food-borne pathogen shared by human being and animals which has a strong resistance to the adverse environment,and widely exists in various food processing environment and easily causes severe food safety problems. LM is a kind of intracellular parasitic bacterium,its virulence factor internalin A（ InlA） and internalin B（ InlB） are deemed to play important roles in LM penetrating the host barrier,invading the host cell and the transmission between cells. This paper adopted homologous recombination method t...