期刊论文

Initial Study on Bollworm (Helicoverpa armigera) as an Infection Model of Listeria monocytogenes

中文

微生物学杂志

1005-7021

2019

39

6

47-55

10.3969/j.issn.1005-7021.2019.06.006

国家自然科学基金项目(31571931) 遗传调控与整合生物学湖北省重点实验室开放课题项目(GRIB201709) 华中师范大学中央高校基本科研业务费资助项目(CCNU18TS024)。

本校为第一机构

研究单核细胞增生李斯特菌(Listeria monocytogenes,简称Lm)对棉铃虫(Helicoverpa armigera)的致病性,探讨棉铃虫作为Lm感染模型的可行性。用野生株EGDe(中毒)、PrfA缺失株(EGDeΔprfA,弱毒)和PrfA 组成型高表达株(EGDeΔprfA + pERL3-prfA*,高毒)腹腔微量注射4龄棉铃虫幼虫,计算半数致死量(LD_(50))和虫体的载菌量,同时观测棉铃虫中肠细胞的病理变化以及血细胞包囊作用和数目。实验结果显示:①EGDe、EGDeΔprfA 和 EGDeΔprfA + pERL3-prfA * 的 LD_(50)分别为2.56 × 10~3 cfu/mL、1. 98 × 10~6 cfu/mL 和6. 63 × 10~2 cfu/ mL。②感染Lm后,EGDe和EGDeΔprfA + pERL3-prfA *在棉铃虫体内各部...

The feasibility of using bollworm (Helicoverpa armigera) (Ha) as infection model of Listeria monocytogenes (Lm) to study the pathogenicity of Lm was investigated. Three different virulent Lm strains, i. e. the wild strain EGDe (middle virulent), PrfA deletion mutant EGDeΔprfA (attenuated),and PrfA constitutively high expression mutant EGDeΔprfA + pERL3-prfA * (highly virulent) were intraperitoneally micro-injected into the 4th Ha larvae instar to calculate the 50% lethal dose (LD_(50)) of Lm strains against Ha larvae,and the burden of bacteri...