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Structural proteins of Helicoverpa armigera densovirus 2 enhance transcription of viral genes through transactivation

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成果类型:
期刊论文
作者:
Xu, Pengjun;Yuan, He;Yang, Xianming;Graham, Robert I.;Liu, Kaiyu;...
通讯作者:
Wu, Kongming
作者机构:
[Yuan, He; Xu, Pengjun; Wu, Kongming; Yang, Xianming] Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, 2 West Yuan Ming Yuan Rd, Beijing 100193, Peoples R China.
[Xu, Pengjun] Chinese Acad Agr Sci, Tobacco Res Inst, 11 Ke Yuan Jing Si Rd, Qingdao, Peoples R China.
[Graham, Robert I.] Harper Adams Univ, Crop & Environm Sci, Edgmond, Shrops, England.
[Liu, Kaiyu] Cent China Normal Univ, Coll Life Sci, 152 Luo Yu Rd, Wuhan, Peoples R China.
通讯机构:
[Wu, Kongming] C
Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, 2 West Yuan Ming Yuan Rd, Beijing 100193, Peoples R China.
语种:
英文
关键词:
Luciferase Activity;Neural Network Promoter Prediction;Internal Renilla Luciferase Control;HaDV2 Genome;Opie2 Promoter
期刊:
Archives of Virology
ISSN:
0304-8608
年:
2017
卷:
162
期:
6
页码:
1745-1750
基金类别:
This research was supported by Science Fund for Creative Research Groups of the National Science Foundation of China (Grant No. 31321004), the National Natural Science Foundation of China (Grant No. 31401752).
机构署名:
本校为其他机构
院系归属:
生命科学学院
摘要:
Herein, we report the identification of putative promoters for the non-structural proteins (NS) and capsid structural proteins (VP) of Helicoverpa armigera densovirus (HaDV2) as well as a potential mechanism for how these promoters might be regulated. For the first time, we report that VP is able to transactivate the VP promoter and, to a lesser degree, the NS promoter in densoviruses. In addition to this, another promoter-like sequence designated P2, when co-transfected with the VP gene, enhanced luciferase activity by approximately 35 times compared to a control. This suggests that there are...

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