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Amorphous metal-organic frameworks on PtCu hydrogels: Enzyme immobilization platform with boosted activity and stability for sensitive biosensing

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成果类型:
期刊论文
作者:
Huang, Jiajia;Jiao, Lei;Xu, Weiqing;Wang, Hengjia;Sha, Meng;...
通讯作者:
Chengzhou Zhu
作者机构:
[Xu, Weiqing; Huang, Jiajia; Zhu, Chengzhou; Sha, Meng; Jiao, Lei; Gu, Wenling; Wang, Hengjia; Wu, Zhichao] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensing Technol, Key Lab Pesticide & Chem Biol,Minist Educ, Wuhan 430079, Peoples R China.
[Hu, Liuyong] Wuhan Inst Technol, Sch Mat Sci & Engn, Wuhan 430205, Peoples R China.
通讯机构:
[Chengzhou Zhu] K
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, PR China
语种:
英文
关键词:
*Biosensing;*Enzyme immobilizations;*Metal-organic frameworks;*Noble metal hydrogels;*Organophosphorus pesticides
期刊:
Journal of Hazardous Materials
ISSN:
0304-3894
年:
2022
卷:
432
页码:
128707
基金类别:
CRediT authorship contribution statement Jiajia Huang: Investigation, Data curation, and Writing – original draft. Lei Jiao: Visualization and Writing. Weiqing Xu, Hengjia Wang, Meng Sha, and Zhichao Wu: Formal analysis and Validation. Wenling Gu and Liuyong Hu: Writing – review & editing. Chengzhou Zhu: Conceptualization, Methodology, Supervision, acquisition, Writing – review & editing, Project administration.
机构署名:
本校为第一机构
院系归属:
化学学院
摘要:
Cell-free enzymatic catalysis (CFEC) is emerging biotechnology that simulates biological transformations without living cells. However, the high cost of separation and preparation of the enzyme has hindered the practical application of the CFEC. Enzyme immobilization technologies using solid supports to stabilize enzymes have been regarded as an efficient strategy to address this issue. Nevertheless, the activity and stability of the immobilized enzymes are still crucial challenges for working in vitro. Herein, an enzyme immobilization platform is developed by using PtCu hydrogels coated with ...

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