3-Nitrosalicyl N-alkylamide was found to be an inhibitor different from antimycin A not only in its inhibitory nature but also in many other aspects. This difference indicated that the 11 kDa component, which was identified as the antimycin A (AA) binding factor in the QH 2 :cytochrome c reductase of Rhodopseudomonas sphaeroides by Wilson et al. ((1985) J. Biol. Chem. 260, 10288-10292) using the radioactive photoaffinity analogue 3-azidosalicyl N-octadecylamide, was not the genuine binding site of AA. Based on the observations that the 3-azidos...