In cold chain logistics, fresh agricultural products are susceptible to deteriorate due to the passage of time in the distribution process. To reduce the loss of cargo, this research integrates the traditional refrigeration cost into the freshness-keeping cost invested in the process of transportation and unloading goods. We rely on the investment of freshness-keeping cost to reduce the cargo damage cost caused by the distribution process and then propose a new vehicle routing problem (VRP). According to all relevant costs, this research builds a mathematical model with the goal of minimizing ...