期刊论文

中文

环境科学学报

0253-2468

2005

25

6

851-854

10.3321/j.issn:0253-2468.2005.06.024

国家科技攻关项目

本校为第一机构

为了探讨在气态甲醛中暴露后小鼠的脑、心和肝组织蛋白质的氧化损伤程度及其发生机制,用不同剂量的气态甲醛对小鼠进行连续动态染毒处理72 h,用2,4-二硝基苯肼比色法测定小鼠脑、心和肝组织蛋白质的羰基含量,用以判断蛋白质的氧化损伤程度.结果显示:吸入0.68mg·m-3甲醛后,小鼠的脑、心和肝蛋白质的羰基含量下降(P＜0.01,P＜0.01,P＜0.05);吸入3.0mg·m-3甲醛后,小鼠的心和肝的蛋白质羰基含量均显著升高(P＜0.01),而脑的蛋白质羰基含量与对照组相比无显著差异(P＞0.05).以上结果表明,小鼠蛋白质的氧化损伤与气态甲醛的浓度有关,气态甲醛在较低浓度时,不引起小鼠蛋白质的氧化损伤,而在中等浓度时...

In order to explore the degree of protein oxidative damage induced by gaseous formaldehyde in mice and its molecular reaction mechanism, the mice were treated with gaseous formaldehyde of different concentrations (0mg·m -3, 0.68mg.m -3 and 3?mg·m -3) for 72hours and the protein carbonyl content was measured by using spectrophotometric DNPH assay to reflect the degree of protein oxidative damage. The results showed that the protein carbonyl content in brain, heart and liver tissue of mice decreased in the group of 0.68?mg·m -3 (P