期刊论文

Wang, B.

中文

环境科学学报

0253-2468

2008

28

10

1969-1975

10.3321/j.issn:0253-2468.2008.10.005

2004CCA00100:国家重大基础研究项目 20060511002:高等学校博士学科点专项科研基金 211;N0.2007AA201c50,2007AA301C26:湖北省科技攻关计划 20062001018:武汉市科技攻关计划重点项目

本校为第一且通讯机构

从湖北沙隆达农药厂污水处理池的活性污泥中分离到一株能以甲基对硫磷（MP）和对硝基苯酚（PNP）为唯一碳源生长的细菌HS-D36，经生理生化试验和16SrDNA同源性分析，初步鉴定为施氏假单胞菌（Pseudomonasstutzeri）．该菌在3h内对浓度为50mg·L~(-1) MP的降解率为90％；在12h内能将50mg·L~(-1) PNP完全降解．HS-D36在以MP为唯一碳源的无机盐培养基上可以耐受800mg·L~(-1)的MP，在LB培养基上可耐受浓度为2000mg·L~(-1)的MP．同时，克隆了甲基对硫磷水解酶基因mpd，并在E．coli中获得高效表达．重组甲基对硫磷水解酶粗酶液活性达到52．5U．mL~(-1)．

A bacterial strain, HS-D36, capable of degrading methylparathion was isolated from active sludge of a wastewater treatment pond of a pesticide factory in Hubei province. The strain HS-D36 could use methylparathion (MP) and p-nitrophenol (PNP) as the sole carbon source for growth. Analysis of its physiological and biochemical properties along with a homologous comparison of its 16S rRNA gene sequence suggests that the HS-D36 strain belongs to Pseudomonas stiitzeri. Degradation of 50 mg·L -1 MP and PNP reached 90% completion at 3 h and was fully...