期刊论文

The molecular pathways of K562 cell proliferation promoted by low concentrations of formaldehyde

Ding, S.

中文

环境科学学报

0253-2468

2018

38

1

416-424

10.13671/j.hjkxxb.2017.0328

国家自然科学基金面上项目

本校为第一且通讯机构

低浓度甲醛(Formaldehyde,FA)可以促进细胞增殖.本实验选用K562细胞为实验对象,用不同浓度的甲醛溶液处理细胞,探究其中可能存在的分子机制.测定细胞活力及胞内氧化损伤程度后,发现当甲醛浓度为75 μmol·L~(-1)时,细胞增殖率上升(p < 0.05或p < 0.01),Warburg效应中的丙酮酸激酶M2型同工酶(PKM2) 、葡萄糖转运蛋白1(GLUT1) 、乳酸脱氢酶A(LDHA) 、葡萄糖和乳酸含量上升(p < 0.05);此外,细胞周期调控因子Cyclin D-cdk4与转录因子E2F1含量也上升(p < 0.05).同时,细胞内氧化损伤加强,其中活性氧簇(reactive oxygen species,ROS)含量上升(p < 0.01),还原型谷胱甘肽(glutathione,GSH)下降(p < 0.05)...

Previous studies have demonstrated that low concentrations of formaldehyde (FA) can promote cell proliferation. In the present study, K562 cells were exposed to different concentrations of FA, aiming to explore the underlying molecular pathway. The cell viability and levels of intracellular oxidative stress were both determined. As the data shown, 75 μmol•L-1 FA exposure increased the levels of cells proliferation rate (p < 0.05 or p < 0.01), as well as the levels of biomarkers involved in the Warburg effect including pyruvate kinase M2 isoen...