期刊论文

Dibutyl phthalate induces neurobehavioral changes in mice and its relationship with physiological and biochemical indexes

中文

环境科学学报

0253-2468

2019

39

7

2384-2391

10.13671/j.hjkxxb.2019.0033

湖北省大学生创新训练项目（No.201810927035）； 湖北省卫生计生委重点支撑项目（No.WJ2017Z027）； 湖北省高等学校优秀中青年科技创新团队计划项目（No.T201717）；

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探究邻苯二甲酸二丁酯诱导小鼠神经行为学改变及与细胞外调节蛋白激酶(ERK1/2)通路相关蛋白的关联.雄性KM小鼠36只,随机分成4组:生理盐水组、50 mg·kg~(-1)·d~(-1) DBP组、50 mg·kg~(-1)·d~(-1)维生素E(VE)组、DBP+VE组,连续灌胃处理28 d,观察Morris水迷宫结果,检测小鼠脑海马组织的氧化应激(活性氧(ROS)荧光强度、还原型谷胱甘肽(GSH)与丙二醛(MDA)含量) 、脑源性神经营养因子(BDNF) 、磷酸化cAMP反应元件结合蛋白(p-CREB) 、caspase-3水平,Western blot分析ERK1/2及其磷酸化(p-ERK1/2)水平; H&E、Nissl染色及Hoechst 33258荧光染色分析脑组织CA1区病理学变化.结果表明,与对照组比较,5 0 m...

To explore the neurobehavioral changes induced by dibutyl phthalate and its relationship with extracellular regulated protein kinases (ERK1/2) pathway in mice. 36 male KM mice were randomly divided into 4 groups (n = 9): saline group,50 mg·kg~(-1)·d~(-1) DBP group,50 mg·kg~(-1)·d~(-1) vitamin E (VE) group and DBP+VE group. After 28 days of continuous intragastric administration,the results of Morris water maze were observed. And the levels of oxidative stress (reactive oxygen species (ROS),glutathione (GSH),malondialdehyde (MDA)),brain -der...