14-3-3 proteins are phosphoserine-binding proteins that regulate the activities of a wide array of targets via direct protein-protein interactions, and may play an important role in response to biotic and abiotic stresses. In this study, two cDNAs ( designated as Gh14-3-3b and Gh14-3-3c) encoding putative 14-3-3 proteins were isolated from cotton cDNA libraries. Gh14-3-3b gene encodes a protein of 268 amino acids, while Gh14-3-3c gene encodes a protein of 261 amino acids. Real-time RT-PCR analysis revealed that both the Gh14-3-3b and Gh14-3-3c genes were preferentially expressed in roots. The ...