期刊论文

Lu, Jian Quan

英文

中国化学快报：英文版

1001-8417

2007

18

4

441-444

10.1016/j.cclet.2007.01.012

This work was partially supported by the Science Foundation of the National Education Ministry (No. 206096) and the Education Department of Hubei Province (No. Z200522002).

本校为其他机构

An ss-DNA gold chip was prepared based on self-assembly of the thiol-derivatized oligonucleotide, and used for the determination of single-stranded binding protein (SSB) by surface plasmon resonance microscopy (SPR). The experiment results showed that SSB binds ss-DNA with high specificity, and relative signal of SPR response is proportional to the concentration of SSB in the range of 0.1-100 ng/mL with a detection limit (S/N = 3) of 0.07 ng/mL. (c) 2007 Ran Quan Lu. Published by Elsevier B.V....

一个 ss-DNA 金牌薄片被准备基于 thiol-derivatized oligonucleotide 自己组装，并且由表面电浆子回声显微镜学(SPR ) 为搁浅单人赛的有约束力的蛋白质(SSB ) 的决心使用。实验结果证明 SSB 与高特性绑 ss-DNA，并且 SPR 反应的相对信号与在有检测极限的 0.1 100 ng/mL 的范围的 SSB 的集中成正比(S/N = 3 ) 0.07 ng/mL。