期刊论文

Geng, Hui

英文

Fish & Shellfish Immunology

1050-4648

2019

95

287-296

10.1016/j.fsi.2019.10.051

Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21804048]; Fundamental Research Funds for the Central University of Central China Normal University [CCNU16A02044]; Hubei Province Natural Science Key Foundation Project [2014CFA079]

本校为第一且通讯机构

Disulfide bonds are fundamental in establishing Ig structure and maintaining Ig biological function. Here, we analysed disulfide bonds and free cysteine in three grass carp IgM isoforms (monomeric, dimeric/trimeric, and tetrameric IgM) by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). The results revealed that Cys(574) residue status at the C-terminal tail differed substantially in monomeric IgM in comparison with polymeric IgM, Cys(574) was found as free thiol in monomeric IgM, while it formed disulfide linkages in dimeric/trimeric and tetrameric IgM. F...