Cotyledons, each with a 1-2mm petiole at its base, were cut from axenic seedlings and infected with Arobacterium tumefaciens. After 2-3 days of cocultivation, the cotyledon explants were transferred to MS selection medium containing 15mg / L kanamycin and 4.5ing / L 6-BA to induce shoot differentiation. Kanamycin-resistant shoots were subeultured on selection medium with 20-50mg / L kanamycin for 3-6 months for eliminating escaped non-transformants, and then rooted on MS medium containing 25mg/ L kanamycin and 0.1mg/ L NAA. Whole Plants were transplanted into soil and grew in the field. DNA So...
