期刊论文

Li, X.

英文

遗传学报

1673-8527

2007

34

2

151-159

10.1016/S1673-8527(07)60016-2

This work was supported by National Program for Basic Research （973 project） of China （No. 2004CB117304）, the Ministry of Education of China （No. 104130）, National Program for High Technology （863 Project） of China （No. 2005AA220270）, and Na-tional Natural Sciences Foundation of China （No. 30470930）.；

本校为第一且通讯机构

The 14-3-3 protein, highly conserved in all eukaryotic cells, is an important regulatory protein. It plays an important role in the growth, amplification, apoptosis, signal transduction, and other crucial life activities of cells. A cDNA encoding a putative 14-3-3 protein was isolated from cotton fiber cDNA library. The cDNA, designated as Gh14-3-3L (Gossypium hirsutum 14-3-3-like),is 1,029 bp in length (including a 762 bp long open reading frame and 5'-/3'-untranslated regions) and deduced a protein with 253 amino acids. The Gh14-3-3L shares higher homology with the known plant 14-3-3 protein...

14-3-3蛋白以二聚体形式存在于所有真核生物中,是一种高度保守的调节蛋白,在细胞生长、增殖、凋亡、信号转导等生命活动中发挥着重要调控作用.我们在棉纤维cDNA文库中分离克隆到1个基因(cDNA),编码14-3-3蛋白类似物,命名为Gh14-3-3L(Gossypium hirsutum 14-3-3-like).该cDNA长度为1,029 bp,包含762bp开放阅读框,其编码蛋白由253个氨基酸组成.Gh14-3-3L与其他真核生物的14-3-3蛋白具有较高的同源性,并具有14-3-3蛋白的基本结构:二聚体结构域、磷酸化丝氨酸富集识别序列、4个CC结构和1个EF Hand结构.Northern杂交分析显示Gh14-3-3L在棉纤维发育早期优势表达,且在10DPA棉纤维细胞中表达量最高,这表...