期刊论文

中文

武汉大学学报(理学版)

1671-8836

2005

51

6

733-738

10.3321/j.issn:1671-8836.2005.06.017

本校为第一机构

采用余姚某鸭场濒死鸭肝组织悬液,接种鸭胚尿囊腔增殖病毒,蔗糖梯度离心法纯化病毒,电镜观察见直径约20nm的球形病毒粒子,免疫琼脂扩散实验结果显示与鸭细小病毒(duck parvovirus ,DPV)抗血清有明显沉淀线;SDS-PAGE电泳可见3条结构蛋白带,与DPV毒株一致;参照GenBank DPV-ns基因序列979～1 566 bp设计引物,PCR扩增反应获得其目的片段.克隆到载体pMD18-T后测序,该序列与GenBank 中的番鸭细小病毒(Muscovy duck parvovirus,MDPV)以及巴比里鸭细小病毒(Barbarie duck parvovirus,BDPV)的ns基因序列同源性为98%.病鸭肝组织匀浆液雏鸭感染试验显示雏鸭发病症状及剖解病理变化明显,死亡率为75%.利用血清...

An acute and contagious disease broke out in several duck farms in the region of Ningbo of Zhejiang Province in 2004. In the research,the suspension of the dead ducks＇ liver tissues was inoculated into the allantoic cavity of 11-day-old duck embryos. After the purification of virus by the sucrose density gradient centrifugation, a typical Duck Parvovirus（DPV） particle was observed as the size of 20 nm by electron microscopy. In the test of double agar gel immunodiffusion, a line of precipitation was visible. Through SDS-PAGE, three main segm...