期刊论文

中文

中国油料作物学报

1007-9084

2022

44

4

751-761

10.19802/j.issn.1007-9084.2021134

国家自然科学基金（31971814,31571572）； 国家重点研发计划（2016YFD0100202）；

本校为第一机构

植物低磷与低温胁迫应答之间存在基因调控及生理生化适应关联性。MYB-CC家族转录因子PHR1是植物低磷应答核心调控因子,但PHR1是否参与植物低温胁迫应答调控及其扮演的功能还不清楚。本文以BnPHR1过量表达转基因油菜为材料,探究BnPHR1在油菜低温胁迫应答中的调控功能。相比野生型油菜,BnPHR1过量表达转基因油菜株系对低温的耐受性明显提高,叶片萎焉程度减轻。电导率和丙二醛含量分析表明转基因油菜细胞膜损伤程度降低。为了进一步探究BnPHR1在低温胁迫中的作用,从野生型(WT)及BnPHR1过量表达转基因油菜转录组数据中挖掘低温胁迫应答相关差异表达基因,探索BnPHR1影响油菜低温抗性的可能机制。转录...

There is a similarity on gene expression and physiological/biochemical adaptation between the low phosphorus and low temperature stresses in plants.PHR1,one of MYB-CC family transcription factors,is a core regulator of low phosphorus stress in plants.Up to now,the function of PHR1 in low temperature stress in plants is not clear.In this study,the function of BnPHR1 in low temperature was analyzed with BnPHR1 overexpressing transgenic rapeseed.Compared with wild-type rapeseed,the tolerance of BnPHR1 overexpressing transgenic rapeseed was significantly improved.The electrolyge leakage(EL)and mal...