Development of a reversibly switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI)

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成果类型：

期刊论文

作者：

Zhang, Xi;Chen, Xuanze;Zeng, Zhiping;Zhang, Mingshu;Sun, Yujie;...

通讯作者：

Xi, Peng

作者机构：

[Peng, Jianxin; Zhang, Xi] Cent China Normal Univ, Sch Life Sci, Inst Entomol, Wuhan 430079, Peoples R China.

[Zhang, Mingshu; Zhang, Xi; Xu, Pingyong] Chinese Acad Sci, Inst Biophys, Key Lab RNA Biol, Beijing 100101, Peoples R China.

[Zeng, Zhiping; Xi, Peng; Chen, Xuanze] Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China.

[Chen, Xuanze; Sun, Yujie] Peking Univ, Sch Life Sci, Biodynam Opt Imaging Ctr BIOPIC, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China.

通讯机构：

[Xi, Peng] P

Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China.

语种：

英文

关键词：

reversibly switchable fluorescent protein;super-resolution microscopy;Skylan-S;protein engineering;live-cell imaging;SOFI

期刊：

ACS Nano

ISSN：

1936-0851

年：

2015

卷：

期：

页码：

2659-2667

DOI：

10.1021/nn5064387

基金类别：

National Basic Research Program ("973" Program) of ChinaNational Basic Research Program of China [2013CB910103, 2010CB933901, 2011CB809101]; National Instrument Development Special Program [2013YQ03065102]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31170818, 31370851, 61178076, 31327901, 61475010, 31300612]; Chinese Academy of SciencesChinese Academy of Sciences [XDB08030202]; Beijing Natural Science Foundation, ChinaBeijing Natural Science Foundation [7131011]

机构署名：

本校为第一机构

院系归属：

生命科学学院

摘要：

Reversibly switchable fluorescent proteins (RSFPs) can be effectively used for super-resolution optical fluctuation imaging (SOFI) based on the switching and fluctuation of single molecules. Several properties of RSFPs strongly influence the quality of SOFI images. These properties include (i) the averaged fluorescence intensity in the fluctuation state, (ii) the on/off contrast ratio, (iii) the photostability, and (iv) the oligomerization tendency. The first three properties determine the fluctuation range of the imaged pixels and the SOFI sig...

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Development of a reversibly switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI)

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