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Comparative phosphoproteomics reveal new candidates in the regulation of spermatogenesis of Drosophila melanogaster

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成果类型:
期刊论文
作者:
Mao, Bin;Zhang, Wei;Zheng, Ya;Li, Dong;Chen, Meng-Yan;...
通讯作者:
Wang, YF
作者机构:
[Mao, Bin; Li, Dong; Zhang, Wei; Chen, Meng-Yan; Wang, Yu-Feng; Zheng, Ya] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构:
[Wang, YF ] C
Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
语种:
英文
关键词:
Drosophila melanogaster;individualization complex;phosphoproteome;Slmap;spermatogenesis
期刊:
昆虫科学(英文)
ISSN:
1672-9609
年:
2022
卷:
29
期:
6
页码:
1703-1720
基金类别:
We thank Professor Scott L. O'Neill (Monash University, Australia) for providing Dmel Mel flies, Professor Dahua Chen (Institute of Zoology, Chinese Academy of Sciences) for providing bamGal4 fly line, and Beijing Genomics Institute (BGI, Shenzhen, China) for technical assistance in identification and quantitation of phosphopeptides and some data analyses. This work was supported by the National Natural Science Foundation of China (no. 31672352, 31872288). w
机构署名:
本校为第一且通讯机构
院系归属:
生命科学学院
摘要:
The most common phenotype induced by the endosymbiont Wolbachia in insects is cytoplasmic incompatibility, where none or fewer progenies can be produced when Wolbachia-infected males mate with uninfected females. This suggests that some modifications are induced in host sperms during spermatogenesis by Wolbachia. To identify the proteins whose phosphorylation states play essential roles in male reproduction in Drosophila melanogaster, we applied isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic strategy combined with titanium dioxide (TiO2) enrichment to compare the ...

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