期刊论文

Function Analysis of Cotton GhWLIM5 in Cotton Fiber Development

中文

山西农业科学

1002-2481

2023

51

09

974-982

10.3969/j.issn.1002-2481.2023.09.02

2016ZX08009003:国家科技重大专项 32172039:国家自然科学基金

本校为第一机构

LIM结构域蛋白是重要的发育调控因子，常作为肌动蛋白结合蛋白（F-Actin）参与调控细胞骨架建成。为了进一步研究GhWLIM5在棉纤维中的生物学功能，通过研究棉花的遗传转化并获得相应的转基因植株，同时对多株转基因植株进行表型分析，并通过分子试验对棉花LIM蛋白GhWLIM5在棉花纤维发育中的功能进行相关研究。结果表明，与野生型相比，抑制GhWLIM5表达的GhWLIM5 RNAi转基因棉花纤维中肌动蛋白细胞骨架较为稀疏，细胞生长速度降低，成熟纤维较短，纤维强度较弱。通过低速共沉淀检测发现，GhWLIM5促进F-Actin成束的能力与pH环境密切相关，随着pH值的升高，GhWLIM5促进F-Actin成束的能力逐渐下降。...

LIM domain proteins are important development regulatory factors,and often involved as an actin binding protein(F-Action)in regulating cytoskeleton construction.To further explore the biological function of GhWLIM5 in cotton fiber,in this study,corresponding transgenic ...MORE LIM domain proteins are important development regulatory factors,and often involved as an actin binding protein(F-Action)in regulating cytoskeleton construction.To further explore the biological function of GhWLIM5 in cotton fiber,in this study,corresponding transgenic plants were obtained by studying the genetic transfo...