期刊论文

中文

华中师范大学学报(自然科学版)

1000-1190

1991

025

1

66-72

10.19603/j.cnki.1000-1190.1991.01.014

本校为第一机构

胶原酶消化分离豚鼠肾上腺髓质细胞,并在含牛血清白蛋白(5 mg/mL)的缓冲液中进行短时间培养,作为研究儿茶酚胺分泌和神经兴奋——分泌偶联的模型。髓质嗜铬细胞分泌儿茶酚胺与时间呈正相关,回归方程为:Y=1.8889+2.0086×10~(-2)X。在存在Ca~(2+)(2 mmol/L)时,神经节后拟胆碱药硝酸毛果芸香碱促进儿茶酚胺分泌的作用显著加强,分泌量与硝酸毛果芸香碱的浓度呈对数曲线,回归方程:Y=8.1072+5.4457×10~(-1)logX,M受体阻断剂阿托品竞争性抑制硝酸毛果芸香碱的作用。

The primary culture of isolated adrenomedullary cells are prepared by collagenase digestion of guinea pig adrenal gland and maintained in Buffer Ⅱ containing 5 mg/ml bovine serum albumin. The cell suspension Could be a system very helpful in studying cate-cholamine secretion and provided a useful model system for study the excitation—secretion couping in neuron. The isolated medulla cell catecholamine secretion is time—dependent. The regression equation is Y=1.8889+2.0086×10~(-2)Ⅹ. In the presence of Ca~(2+) (4mmol/L). the postganglionic i...