期刊论文

中文

华中师范大学学报(自然科学版)

1000-1190

1997

31

3

328-331

10.19603/j.cnki.1000-1190.1997.03.018

国家自然科学基金

本校为第一机构

生长调节物质对丹参叶片脱分化及根芽分化效应的研究结果表明:(1)0.5-5.0mg/L2,4-D、0.1-0.5mg/L NAA、0.5-1.5mg/L IBA或0.2-1.0mg/L 4PU-30诱导叶片的脱分化率高达97%-100%。(2)0.1-0.5mg/L NAA或0.5-2.0mg/IBA诱导芽分化率为93%-100%;3种细胞分裂素中,4PU-30对叶片芽分化最有效,6-BA和KT次之。(3)随2,4-D、NAA和IAA浓度增大,愈伤组织或再生苗的根分化率也增高。

The present investigation deals with the effect of growth regulating substances on the dedifferentiation and roots and buds differentiation in tissue culture of the blade of salvia miltiorrhiza Bunge.The results were that:(1)The frequency of the dedifferentiation induced by 2,4 D (0.5～5.0 mg/L),NAA (0.1～0.5 mg/L) ,IBA (0.5～1.5 mg/L)or 4PU 30 (0.2～1.0 mg/L) was 97%～100%. (2)The frequency of buds differentiation induced by NAA (0.1～0.5 mg/L), or IBA (0.5～2.0 mg/L) was 93%～100%, among the three cytokinins tested, 4PU 30 was superior to 6 B...