期刊论文

中文

华中师范大学学报(自然科学版)

1000-1190

1998

32

1

77-80

10.19603/j.cnki.1000-1190.1998.01.018

国家“九五”攻关计划；

本校为第一机构

以籼稻H28优良品系为材料,通过胚培养诱发愈伤组织并继代培养.最适的诱导培养基为MS+2 mg/L 2,4-D,在此培养基上产生的愈伤组织具有较强的分化能力.将愈伤组织转移到分化培养基,光照培养17 d后,在MS+0.25 mg/L NAA+1 mg/L BA的分化培养基上,愈伤组织的芽分化率可达40%.在分化培养基上培养10 d后,将愈伤组织转移到无激素或低浓度激素的培养基上,其分化出苗率有明显的提高.

In the present report, conditions of plant regenerations from mature embryo culture of rice ( Oryza sativa L.) in vitro was studied. The results obtained in mature embryo culture showed that MS medium containing 2 mg/L 2,4 D was favorable for forming compact calli, shoot buds were regenerated on the surface of the calli with frequency of 40% when the calli were transferred to MS medium with 0.25 mg/L NAA and 1 mg/L BA un...