期刊论文

中文

华中师范大学学报(自然科学版)

1000-1190

1999

33

1

108-111

10.19603/j.cnki.1000-1190.1999.01.022

国家自然科学基金

本校为第一机构

不同光质和温度对丹参叶片启动率、愈伤组织和芽形成率均有不同的效应。以绿光诱导芽形成频率最高,达96%。30℃条件下芽的诱导频率提高,可达99%。一般,从丹参叶片接种到植株再生只需30-40d;每块0.5cm~(2)的叶片可产生20-40个芽。试管苗移栽后的存活率为90%-95%。

There was different effect with various wavelengths of light and temperature on initiation, callus and bud formation frequency of blade in Salvia miltiorrhiza Bunge . Under green light, the frequency of buds induction was the highest, up to 96%. The frequency of buds induction was increased in 30℃, being 99%. In general, it only needed 30～40 d from blade inoculation to plant regeneration. A 0.5 cm 2 blade could produce 20～40 buds. Survival rate of plantlets was up...