In this paper, it described the problem of manipulation technology for breeding rice by anther culture and selection of somatic mutants. It was important for callus induction and shoot differentiation to choose better starting materials and a suitable medium in rice anther culture. Some elements (such as casein hydrolysate, nucleolide, AgNO 3,etc.)added in the medium might increase the frequency of greenshoot differentiation in rice anther culture. Somatic variation can provide a lot of materials selected for crop breeding. It expounds the proc...