期刊论文

中文

华中师范大学学报(自然科学版)

1000-1190

2006

40

3

407-411

10.19603/j.cnki.1000-1190.2006.03.025

30570799:国家自然科学基金 20056002058:湖北省武汉市科技攻关计划

本校为其他机构

为了研究甲醛致昆虫细胞DNA-蛋白质的交联作用(DNA-protein crosslinks,DPC)和DNA的断裂作用,以斜纹夜蛾SL-1细胞为材料,采用KCl-SDS沉淀法和彗星实验来检测液态甲醛染毒后SL-1细胞中DPC的含量及DNA的断裂效应.KCl-SDS沉淀法的结果表明,低浓度的液态甲醛(25μmol/L,125μmol/L)不能引起DPC,较高浓度(625μmol/L)可以引起明显的DPC(P

To study the effect of formaldehyde inducing DNA-protein crosslinks （DPC） and DNA single strand break （DSSB） in insect ceils, this study took Spodoptera litura SL-1 cells as experimental material and made use of KC1-SDS assay and comet assay to determine the amount of DNA-protein crosslinks and effect of DNA damages induced by liquid formaldehyde. From the KC1-SDS assay results formaldehyde can not cause DPC at low levels （25 μmol/L,125 μmol/L）, but at higher levels （625μmol/L） can cause DPC obviously （P〈0.01）. According to comet ...