For study on the interaction between o-nitrophenol and deoxgribonucleic acid, ultraviolet-visble spectrophotometry, fluorescence spectrophotometry and quantum chemistry were used to study the interaction between o-nitrophenols and deoxgribonucleic acid in pH 7.42 sodium phosphate buffer solution. The DNA adducts can be formed in opening chain process, which can shift the peaks and increase the intension of absorption and fluorescence spectra. However, such interaction in 37℃ is weak than in opening chain process, the theoretical calculation in...