Non-viral gene vector is one of hot topics of gene therapy. Based on a long time our research on calixarene chemistry and with the progress of artificial virus and gemini surfactant, it is designed to synthesize a new glyco calixarene(GGC[4]) as gene carrier. It is observed that glycocalix[4]arene and DNA are mixed to afford a nano supramolular complexes in TEM images. In vitro transfection assays show that glycocalix [4]arene(GGC[4]) have a high tranfection efficiency. It is very important academic and practice significance for this research to make pro...