The interaction between （Z）-4-benzylidene-1-（4-chlorophenyl）-2-propylamino- 1H-imidazol-5（4H）-one（BPHS） and bovine serum albumin （BSA） was studied by florescence spectroscopy and UV-vis absorption spectroscopy. The fluorescence intension of BSA could be quenched strongly by BPH5 in the experiment and the mechanism is a dynamic quenching procedure. The values Of binding sites n and apparent binding constant KB were measured by fluorescence quenching method. The thermodynamics parameter AH, AG, AS were calculated. The results indicated ...
