期刊论文

中文

华中师范大学学报(自然科学版)

1000-1190

2008

42

2

262-266

10.19603/j.cnki.1000-1190.2008.02.026

国家自然科学基金项目资助（30470930）；

本校为第一机构

采用TCA-丙酮沉淀法,改良优化了棉花蛋白质组研究中的双向电泳技术.通过对根和子叶全细胞蛋白的提取、蛋白的溶解、胶条的选择及电泳等环节的优化,得到了重复性很高、分辨率很好的蛋白质图谱.进一步对5 d苗龄的棉花进行4℃低温处理12 h后,提取子叶的蛋白质,利用双向电泳技术分离全细胞蛋白.并用PDQuest软件分析比较棉花子叶在低温处理下的蛋白表达谱,得到了49个有显著差异的蛋白点.其中,低温处理上调表达的蛋白点有27个,减弱表达的蛋白点有22个,推测这些差异蛋白点可能与低温胁迫相关.

The proteins of cotton （Gossypium hirsutum） were extracted by TCA-acetone method and the technique of two-dimensional electrophoresis in cotton proteomics was improved. Some satisfied pictures were obtained through optimizing many key procedures, such as sample preparation, sample dissolution, IPG stripe, IEF electrophoresis, SDS-PAGE. Then proteins from cold treated cotton cotyledon for 12 hours were extracted and separated by 2-DE. A large quantity of protein spots appeared in pictures. PDQuest software was used to analyze the pictures and ...