期刊论文

中文

华中师范大学学报(自然科学版)

1000-1190

2011

45

2

272-278

10.19603/j.cnki.1000-1190.2011.02.023

基金项目：国家转基因重大专项（2009ZX08009-117B）.

本校为其他机构

从棉花纤维cDNA文库中分离了3个新的基因,对其编码的蛋白质结构特点的分析表明:这3个基因属于苯基苯乙烯酮合酶(chalcone synthase,CHS)类基因(分别命名为GhCHS2, GhCHS3和GhCHS4)．在核苷酸水平上,GhCHS2与GhCHS3的cDNA序列同源性达到73%, GhCHS3与GhCHS4的cDNA序列同源性达到67%,而GhCHS2与GhCHS4的cDNA序列同源性达到63%.在蛋白质水平上,GhCHS2与GhCHS3达到85%同源性,GhCHS2与GhCHS4达到 60%同源性,而GhCHS3与GhCHS4同源性达到58%.荧光定量RT-PCR分析表明:GhCHS2、 GhCHS3和GhCHS4都在棉纤维中优势表达,而在其他组织中表达量较低,结果表明它们可能在棉花纤维发育过程中起作用,

In this study, three novel genes were isolated from cotton fiber CDNA library. The isolated genes encode putative chalcone synthases （CHS） （designated GhCHS2, GhCHS3 and GhCHS4, respectively）. At cDNA level, GhCHS2 has 73% identity with GhCHS3, and GhCHS3 shares 67%o identity with GhCHS4, while GhCHS2 displays 63% identity with GhCHS4. At protein level, GhCHS2 shares 85% identity with GhCHS3 protein, and GhCHS2 shows 60% identity with GhCHS4, while GhCHS3 shares 58% identity with GhCHS4. Real-time quantitative RT-PCR analysis revealed that ...