One-pot bioinspired synthesis of all-inclusive protein-protein nanoflowers for point-of-care bioassay: detection of E. coli O157:H7 from milk

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Ye, Ranfeng;Zhu, Chengzhou;Song, Yang;Song, Junhua;Fu, Shaofang;...

通讯作者：

Du, Dan;Lin, Yuehe

作者机构：

[Ye, Ranfeng; Li, He; Du, Dan; Yang, Xu] Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.

[Ye, Ranfeng; Li, He; Du, Dan; Yang, Xu] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.

[Ye, Ranfeng; Zhu, Chengzhou; Li, He; Lu, Qian; Song, Junhua; Du, Dan; Song, Yang; Fu, Shaofang; Lin, Yuehe; Du, D; Lin, YH] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.

[Zhu, Mei-Jun] Washington State Univ, Sch Food Sci, Pullman, WA 99164 USA.

通讯机构：

[Du, Dan] C

[Du, D; Lin, YH] W

Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.

Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.

Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.

语种：

英文

期刊：

Nanoscale

ISSN：

2040-3364

年：

2016

卷：

期：

页码：

18980-18986

DOI：

10.1039/c6nr06870g

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21575047]; WSU startup fund; China Scholarship CouncilChina Scholarship Council [201406770010]

机构署名：

本校为第一且通讯机构

院系归属：

生命科学学院

化学学院

摘要：

Protein-protein conjugates play a vital role in bioassays with their inherent functions of biological recognition and signal amplification. Herein, a one-pot green method for synthesis of all-inclusive protein-protein nanoflowers has been developed. The protein-protein nanoflowers integrate both essential functions of biological recognition and signal amplification, and they were used as ideal signal labels for the sensitive point-of-care detection of Escherichia coli O157:H7. Especially noteworthy, the prepared Con A-invertase-CaHPO4hybrid nanoflowers simultaneously loaded sufficient invertas...

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One-pot bioinspired synthesis of all-inclusive protein-protein nanoflowers for point-of-care bioassay: detection of E. coli O157:H7 from milk

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