Based on natural conversation corpus, this paper investigates the location distribution,interactive function and influencing factors of the discourse marker “nayou”.The results show that the discourse marker “nayou” has a strong preference for the position of turn or discourse boundaries, showing non-primordial pragmatic characteristics in the sequence. There are four main interactive functions of “nayou” in speech and communication:(1) Discursive negation;(2)Discourse repair;(3) Turn-taking;(4) Topic termination. There are two main facto...