期刊论文

中文

中国实验方剂学杂志

1005-9903

2016

22

2

97-102

10.13422/j.cnki.syfjx.2016020097

国家基础科学人才培养基金项目（J1103514） 2015年华中科技大学自主创新研究基金前沿探索项目（2015TS091）

本校为其他机构

目的:研究玛咖脂溶性提取物对睡眠剥夺造成的小鼠学习记忆力下降的影响,并对脂溶性成分进行分析。方法:将48只雄性昆明种小鼠随机分为空白组,睡眠剥夺组,玛咖脂溶性提取物低、高剂量(60,200 mg·kg~(-1))组,连续ig给药16 d。实验第13天采用小平台水环境法制备小鼠睡眠剥夺模型,以通道式水迷宫实验测试小鼠睡眠剥夺24,48,72 h的学习记忆能力,观察睡眠剥夺72 h后小鼠脑组织病理学变化并检测脑组织超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px),脂质过氧化物丙二醛(MDA)含量。用气相色谱-质谱仪(GC-MS)和高效液相色谱仪(HPLC)对玛咖脂溶性成分进行检测。结果:与空白组相比,睡眠剥夺组小鼠水迷宫...

Objective:To investigate the effect of Lepidium meyenii lipid-soluble extract on the decline of the learning and memory ability of sleep deprived mice, and analyze the lipid-soluble extract. Method:Totally 48 male Kunming mice were randomly divided into 4 groups, large platform control group, sleep deprivation group, low dose group of L. meyenii lipid-soluble extract (60 mg·kg~(-1)) and high dose group of L. meyenii lipid-soluble extract (200 mg·kg~(-1)), they were administrated by lavage for 16 days. On the 13th day, a small platform was use...