BackgroundGuided gaze is the instructor's gaze towards teaching materials to guide students' attention, and it plays a vital role in enhancing video-based education. The duration of guided gaze, indicating how long instructors focus on teaching materials, varies based on the lecture design. Nevertheless, the impact of varying durations of guided gaze, especially concerning students' prior knowledge, remains inadequately understood.ObjectivesThis study investigates the influence of the instructor's guided gaze duration and students' prior knowledge on learning performance and affective experien...