A novel cheap and simple amperometric glucose biosensor, based on the electrode modified with the Ni/Al layered double hydroxide (LDH) nanoflakes and chitosan (CHT), without glucose oxidase, is presented. The glucose biosensor based on monodispersed high active Ni/Al-LDH nanoflakes and CHT exhibits an appropriate linear range of 0.01-10 mM and good operational stability. The amperometric sensor shows a rapid response at the potential value 0.48 V. In addition, optimization of the biosensor construction, the effects of the applied potential, the...