期刊论文

Variances in morphology of the rat model similar to aged human diabetes mellitus

中文

中国老年学杂志

Chinese Journal of Gerontology

1005-9202

2010

30

15

2132-2136

10.3969/j.issn.1005-9202.2010.15.023

2006福建中医学院中西医结合研究院陈可冀发展基金会研究课题(CKJ2006006),2008年福建中医学院中西医结合研究院陈可冀发展基金会研究课题(CKJ2008040),2008年福建中医学院校管课题(X2007016)； 福建省教育厅F5类项目(2008F5028)；

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目的 采用D-半乳糖加高脂高糖乳剂及链脲佐菌素(STZ)构建类似老年人糖尿病的大鼠动物模型,观察其胰腺形态学变化.方法 选用健康Wistar雌性大鼠,20只灌胃生理盐水为正常对照组;20只采用腹腔注射D-半乳糖40 d为衰老模型组;另25只则采用腹腔注射D-半乳糖40 d并灌胃高脂高糖乳剂30 d后腹腔注射STZ(体重≤200 g按30 mg/kg,体重＞200 g者按体表面积进行计算),制备衰老糖尿病模型大鼠,筛选空腹血糖≥11.1 mmol/L者为成模鼠,上述各组大鼠再继续灌胃生理盐水10 d后,各组随机取10个样本以免疫组化、光镜和电镜分别观察大鼠胰岛诱导型一氧化氮合酶(iNOS)表达、胰腺细胞凋亡、胰岛形态及胰岛细胞和腺泡细胞超...

Objective To observe the variances in morphology of the rat model similar to aged human diabetes mellitus （DM）.Methods Healthy female Wistar rats were chosen.20 rats were intragastric administrated with physiological saline to build the normal control group;20 rats were intraperitoneal injected with D-galactose for 40 days to set up the aged model group;and 25 rats were intraperitoneal injected with D-galactose for 40 days,intragastric administrated with enriched diet for 30 days since injection with D-galactose 11 days,then intraperitoneal i...