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A novel aptamer-based online magnetic solid phase extraction method for simultaneous determination of urinary 8-hydroxy-2′-deoxyguanosine and monohydroxylated polycyclic aromatic hydrocarbons

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成果类型:
期刊论文
作者:
Gan, Haijiao;Xu, Hui*
通讯作者:
Xu, Hui
作者机构:
[Gan, Haijiao; Xu, Hui] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构:
[Xu, Hui] C
Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Hubei, Peoples R China.
语种:
英文
关键词:
8-Hydroxy-2'-deoxyguanosine;Aptamer;High performance liquid chromatography-mass spectrometry;Online magnetic solid phase extraction;Polycyclic aromatic hydrocarbon;Urine
期刊:
Talanta
ISSN:
0039-9140
年:
2019
卷:
201
页码:
271-279
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21675058]; Hubei Province Natural Science Fund Project outstanding youth project, China [2017CFA075]; self determined research funds of CCNU from the colleges' basic research and operation of MOE [CCNU18TS006]; 111 ProjectMinistry of Education, China - 111 Project [B17019]
机构署名:
本校为第一且通讯机构
院系归属:
化学学院
摘要:
In this work, an innovative aptamer-based magnetic adsorbent (Fe3O4@PDA-aptamer MNPs) was prepared by hydrothermal synthesis method followed by the surface functionalization of nanoparticles. After fixing in a steel stainless tube as sorbent of magnetic solid phase extraction (MSPE), an online magnetic solid phase extraction-high performance liquid chromatography-mass spectrometry (online-MSPE-HPLC-MS) method was developed and applied for the determination of 8-hydroxy-2′-deoxyguanosine (8-OHdG) and monohydroxylated polycyclic aromatic hydroca...

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