The title compound, C25H20N6O3·CHCl3, a rigid glycoluril derivative, is an inter-mediate for the synthesis of mol-ecular tweezers. The crystal structure is stabilized by inter-molecular C - H⋯O and N - H⋯O hydrogen bonds. The chloro-form solvent mol-ecule is disordered over two positions; the site occupancy factors are ca 0.89 and 0.11. © Intern...