期刊论文

中文

环境与健康杂志

1001-5914

2016

33

8

679-682

10.16241/j.cnki.1001-5914.2016.08.005

国家自然科学基金（51136002）；

本校为第一机构

目的探讨PM_（2.5）对小鼠脑组织的急性毒性效应。方法将SPF级雄性Balb/c小鼠48只随机分为6组：对照组（生理盐水组）、生理盐水＋褪黑素组、4μg/d PM_（2.5）组、20μg/d PM_（2.5）组、100μg/d PM_（2.5）组、100μg/d PM_（2.5）＋褪黑素组,每组8只。各染毒组分别以0.1、0.5、2.5 mg/ml PM_（2.5）溶液进行气道滴注,每只小鼠每天滴注40μl,连续7 d;生理盐水＋褪黑素组、100μg/d PM_（2.5）＋褪黑素组每天以1.0 mg/ml褪黑素灌胃,灌胃剂量为10 mg/（kg·d）,连续7 d。检测脑组织中活性氧自由基（ROS）、丙二醛（MDA）和还原型谷胱甘肽（GSH）的含量,并采用ELISA法检测脑组织中白细胞介素-4（...

Objective To evaluate the acute toxic effect in mice brain after seven days of exposure to PM_（2.5）. Methods Balb/c mice were randomly divided into six groups, eight in each： control group（saline group）, saline＋melatonin（MT） group,4 μg/d PM_（2.5）group, 20 μg/d PM_（2.5）group, 100 μg/d PM_（2.5）group and 100 μg/d PM_（2.5）＋MT group.PM_（2.5）exposure was conducted through intratracheal instillation and MT treatment was conducted through gavage, for seven consecutive days.Saline＋MT group and 100μg/d PM_（2.5）＋MT group were g...