期刊论文

Li, AX

中文

光谱学与光谱分析

1000-0593

2003

23

5

1002-1004

10.3321/j.issn:1000-0593.2003.05.049

本校为第一且通讯机构

研究了显色剂2-(2,3,5-三氮唑偶氮)-5-甲氨基苯甲酸(简称TZADMAB, 以下简称TZ)与Cu(Ⅱ)的显色反应. 在一定浓度的KNO3介质中, pH=2.5, 室温条件下, 与Cu(Ⅱ)形成蓝紫色络合物, 其组成比为Cu(Ⅱ)∶TZADMAB=1∶2, 稳定常数K=1.0×1012, 最大吸收波长为480 nm. 在此条件下, TZ-Cu(Ⅱ)络合物与脱氧核糖核酸能迅速生成超分子化合物, 使化合物的吸光度迅速降低, 因此利用Cu(Ⅱ)-TZ络合物吸光度降低的原理可以检测脱氧核糖核酸[1]. 脱氧核糖核酸质量浓度在0.02～0.12 mg·mL-1范围内服从比尔定律.

In a potassium nitrate medium, the color reaction between copper(II) and a new developer of 2-(2-3-5-triazolyazo)-5-dimethylarninobenzoic acid (TZADMAB) was studied. At pH 2.5 T7 reacts on copper(II)at room temperature to form a blue violet complex. The mole ratio of this complex is copper(II):T7=1:2. Its absorption maximum lies at 480 nm and its stability constant (beta) is 1.0 x 10(12). Under the same condition, TZ-Cu(II) complex and DNA interact rapidly to form a supermolecular complex, resulting in a rapid decrease in absorption of the complex. So, DNA can be determined with little content...