The specific and rapid labeling of cell surface proteins with recombinant FKBP-fused fluorescent proteins

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Zhang, Xi;Deng, Yongqiang;Chang, Hao;Ji, Chen;Zhang, Mingshu;...

通讯作者：

Peng, Jianxin

作者机构：

[Peng, Jianxin; Zhang, Xi] Cent China Normal Univ, Sch Life Sci, Inst Entomol, Wuhan 430079, Peoples R China.

[Deng, Yongqiang; Xu, Tao; Ji, Chen] Chinese Acad Sci, Inst Biophys, Natl Key Lab Biomacromol, Beijing 100101, Peoples R China.

[Chang, Hao] Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China.

[Zhang, Mingshu; Xu, Pingyong] Chinese Acad Sci, Inst Biophys, Lab Interdisciplinary Res, Beijing 100101, Peoples R China.

通讯机构：

[Peng, Jianxin] C

Cent China Normal Univ, Sch Life Sci, Inst Entomol, Wuhan 430079, Peoples R China.

语种：

英文

关键词：

Cell Surface Protein;Label Efficiency;Ascomycin;Retrograde Trafficking;Dynamic Label

关键词(中文)：

位点特异性;活细胞;荧光蛋白;表面蛋白;标签;重组;标记方法;内吞作用

期刊：

蛋白质与细胞

ISSN：

1674-800X

年：

2014

卷：

期：

页码：

800-803

DOI：

10.1007/s13238-014-0090-8

机构署名：

本校为第一且通讯机构

院系归属：

生命科学学院

摘要：

The presence and abundance of proteins in the plasma membrane (PM) is regulated by exocytosis and endocytosis. Exocytosis is required for the maintenance of cellular homeostasis following stimulation, whereas endocytosis is important for the reuse and degradation of PM and receptor proteins. Through the specific labeling of proteins on the PM, the process of endocytosis can be tracked and monitored in living cells. Several genetic tags and site-specific labeling approaches that involve the coupling of small organic molecules to tag-fused proteins through either self-labeling or enzymatic ligat...

摘要(中文)：

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The specific and rapid labeling of cell surface proteins with recombinant FKBP-fused fluorescent proteins

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