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Artificial Antigen Synthesis and Antibody Production for Di(2-ethylhexyl) phthalate

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成果类型:
期刊论文、会议论文
作者:
Wei Chen-xi;Zhao Wei;Zhang Yan-bo;Yu Xiao-wei;Yuan Jun-lin*
通讯作者:
Yuan Jun-lin
作者机构:
[Yuan Jun-lin; Zhao Wei; Zhang Yan-bo; Wei Chen-xi; Yu Xiao-wei] Huazhong Normal Univ, Lab Environm Sci, Coll Life Sci, Wuhan, Peoples R China.
通讯机构:
[Yuan Jun-lin] H
Huazhong Normal Univ, Lab Environm Sci, Coll Life Sci, Wuhan, Peoples R China.
语种:
英文
关键词:
Di(2-ethylhexyl) phthalate (DEHP);Hapten;Artificial antigen;Antibody
期刊:
2009 3RD INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1-11
年:
2009
页码:
5731-5734
会议名称:
The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)
会议论文集名称:
The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)论文集
会议时间:
2009-06-11
会议地点:
北京
会议赞助商:
IEEE生物医学工程协会
机构署名:
本校为第一且通讯机构
院系归属:
城市与环境科学学院
生命科学学院
摘要:
A hapten di(2-ethylhexyl) 4-aminophthalate (DEHAP) which possesses the structural feature of di(2-ethylhexyl) phthalate (DEHP) was synthesized via esterification and reduction with 4-nitrophthalic acid and 2-ethyl-1-hexanol. The product was confirmed by infrared absorption spectroscopy (IR), 1H nuclear magnetic resonance spectroscopy (1H NMR) and mass spectrometry (MS). Then glutaraldehyde was used as crosslinker to link DEHAP and bovine serum albumin (BSA) to make artificial immunogen DEHAP-BSA. DEHAP was linked to ovalbumin (OVA) through diazotization to prepare coating antigen DEHAP-OVA. Th...

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