To explore the key constructs of multimedia case-based instruction (MCBI), this study developed and validated a questionnaire to examine learners’learning experience with MCBI with tests for reliability and validity. Based on the literature review, the questionnaire was initially designed with 39 questions and was administered to 86 graduate students taking an instructional design course. Item analysis and exploratory factor analysis were conducted sequentially with the questionnaire data, and the statistical results informed the deletion of 13 problematic questions from the overall scal...