期刊论文

Clone and expression pattern analysis of E2 ubiquitin-binding enzyme gene SorUBC in Solanum nigrum L.

中文

东北农业大学学报

Journal of Northeast Agricultural University

1005-9369

2016

19

11

26-36,45

黑龙江省教育厅面上项目(12531204,12531178)； “植物生物学”黑龙江省高校重点实验室开放课题(ZK201205)； 哈尔滨师范大学青年学术骨干资助计划项目(KGB201218)；

本校为其他机构

对龙葵Sor UBC基因克隆并分析其在多种非生物胁迫处理下表达特性,为E2泛素结合酶在植物逆境胁迫中应用提供理论依据。试验采用电子克隆结合RT-PCR方法从龙葵中克隆得到泛素E2结合酶UBC基因编码区序列,命名为Sor UBC,登陆Gen Bank,编号:KU233684。Sor UBC基因编码区全长888 bp,编码295个氨基酸。进化树分析显示,该基因编码氨基酸序列与马铃薯、番茄同源性最高,为95%,其次是烟草,为91%。该蛋白在第266~284氨基酸位具有1个跨膜螺旋区域,泛素化位点预测表明其含有8个泛素化位点。利用荧光定量PCR技术分析Sor UBC基因在龙葵各器官表达特征和不同非生物胁迫处理下(干旱、盐、碱、高温和低温)根部和叶片...

In this research SorUBC gene from Solanum nigrum L. was cloned and the expression pattern under different abiotic stresses were detected. This would help us to predict the characteristics of E2 ubiquitin binding enzyme and provide theoretical basis for the application of E2 ubiquitin binding enzyme in plant stress. The research cloned E2 ubiquitin binding enzyme UBC gene full-length coding region sequence by electronic cloning and RT-PCR method from Solanum nigrum L., named SorUBC,GenBank accession number： KU233684. The coding region sequence ...