Signal-on electrochemiluminescence of biofunctional CdTe quantum dots for biosensing of organophosphate pesticides
作者：
Liang, Han;Song, Dandan;Gong, Jingming*
期刊：
Biosensors and Bioelectronics ,2014年53:363-369 ISSN：0956-5663
通讯作者：
Gong, Jingming
作者机构：
[Song, Dandan; Gong, Jingming; Liang, Han] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Gong, Jingming] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Acetylcholinesterase;Dissolved oxygen;Electrochemiluminescence;Organophosphate pesticides;Quantum dots;Signal-on model
摘要：
A new, highly sensitive and selective ECL assay biosensor based on target induced signal on has been developed for the detection of organophosphate pesticides (OPs), whereby the smart integration of graphene nanosheets (GNs), CdTe quantum dots (CdTe QDs), and acetylcholinesterase (AChE) enzymatic reaction yields a biofunctional AChE-GNs-QDs hybrid as cathodic ECL emitters for OPs sensing. The electrochemically synthesized GNs were selected as a supporting material to anchor CdTe QDs, exhibiting a significantly amplified ECL signal of QDs. On the basis of the effect of OPs on the ECL signal of AChE-QDs-GNs modified glassy carbon electrode (GCE), a highly sensitive GNs-anchored-QDs-based signal-on ECL biosensor was developed for sensing OPs, combined with the enzymatic reactions and the dissolved oxygen as coreactant. The conditions for OPs detection were optimized by using methyl parathion (MP) as a model OP compound. Under the optimized experimental conditions, such a newly designed system shows remarkably improved sensitivity and selectivity for the sensing of OPs. The detection limit was found to be as low as about 0.06ngmL-1 (S/N=3). Toward the goal for practical applications, the resulting sensor was further evaluated by monitoring MP in spiked vegetable samples, showing fine applicability for the detection of MP in real samples. © 2013 Elsevier B.V.
语种：
英文
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Layer-by-layer assembled multilayer films of exfoliated layered double hydroxide and carboxymethyl-β-cyclodextrin for selective capacitive sensing of acephatemet
作者：
Gong, Jingming* ;Han, Xinmei;Zhu, Xiaolei;Guan, Zhangqiong
期刊：
Biosensors and Bioelectronics ,2014年61:379-385 ISSN：0956-5663
通讯作者：
Gong, Jingming
作者机构：
[Gong, Jingming; Han, Xinmei; Zhu, Xiaolei; Guan, Zhangqiong] Cent China Normal Univ, Coll Chem, Inst Environm Chem, Key Lab Pesticide & Chem Biol Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Gong, Jingming] C;Cent China Normal Univ, Coll Chem, Inst Environm Chem, Key Lab Pesticide & Chem Biol Minist Educ, Wuhan 430079, Peoples R China.
关键词：
Capacitive sensor;Carboxymethyl-beta-cyclodextrin;Exfoliated layered double hydroxide;Layer-by-layer assembly;Organophosphate pesticides
摘要：
Novel organic-inorganic hybrid ultrathin films were fabricated by alternate assembly of cationic exfoliated Mg-Al-layered double hydroxide (LDH) nanosheets and carboxymethyl-β-cyclodextrin (CMCD) as a polyanion onto a glassy carbon electrode (GCE) via a layer-by-layer (LBL) approach. The multilayer films were then characterized by means of X-ray powder diffraction (XRD), infrared spectroscopy (IR), and scanning electron microscopy (SEM). These films were found to possess a long range stacking order in the normal direction of the substrate with a continuous and uniform morphology. Its electrochemical performance was systematically investigated. Our results demonstrate that such a newly designed (LDH/CMCD)n multilayer film, combining the individual properties of CMCD (a high supramolecule recognition and enrichment capability) together with LDH nanosheets (a rigid inorganic matrix), can be applied to a sensitive, simple, and label-free capacitive detection of acephatemet (AM). Molecular docking calculations further disclose that the selective sensing behavior toward AM may be attributed to the specific binding ability of CMCD to AM. Under the optimized conditions, the capacitive change of AM was proportional to its concentration ranging from 0.001 to 0.10μgmL-1 and 0.1 to 0.8μgmL-1 with a detection limit 0.6ngmL-1 (S/N=3). Toward the goal for practical applications, this simple probe was further evaluated by monitoring AM in real samples. © 2014 Elsevier B.V.
语种：
英文
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Biosensor based on acetylcholinesterase immobilized onto layered double hydroxides for flow injection/amperometric detection of organophosphate pesticides
作者：
Gong, Jingming* ;Guan, Zhangqiong;Song, Dandan
期刊：
Biosensors and Bioelectronics ,2013年39(1):320-323 ISSN：0956-5663
通讯作者：
Gong, Jingming
作者机构：
[Song, Dandan; Gong, Jingming; Guan, Zhangqiong] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.
通讯机构：
[Gong, Jingming] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.
关键词：
Acetylcholinesterase;Biosensor;Flow injection/amperometric analysis;Layered double hydroxides;Methyl parathion
摘要：
We developed a highly sensitive flow injection/amperometric biosensor for the detection of organophosphate pesticides (OPs) using layered double hydroxides (LDHs) as the immobilization matrix of acetylcholinesterase (AChE). LDHs provided a biocompatible microenvironment to keep the bioactivity of AChE, due to the intrinsic properties of LDHs (such as a regular structure, good mechanical, chemical and thermal stabilities, and swelling properties). By integrating the flow injection analysis (FIA) with amperometric detection, the resulting AChE-LDHs modified electrode greatly catalyzed the oxidation of the enzymatically generated thiocholine product, and facilitated the detection automation, thus increasing the detection sensitivity. The analytical conditions for the FIA/amperometric detection of OPs were optimized by using methyl parathion (MP) as a model. The inhibition of MP was proportional to its concentration ranging from 0.005 to 0.3μgmL-1 and 0.3 to 4.0μgmL-1 with a detection limit 0.6ngmL-1 (S/N=3). The developed biosensor exhibited good reproducibility and acceptable stability. © 2012 Elsevier B.V..
语种：
英文
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Layered titanate nanosheets as an enhanced sensing platform for ultrasensitive stripping voltammetric detection of mercury(II)
作者：
Yuan, Shuang;Peng, Dinghua;Song, Dandan;Gong, Jingming*
期刊：
Sensors and Actuators B-Chemical ,2013年181:432-438 ISSN：0925-4005
通讯作者：
Gong, Jingming
作者机构：
[Peng, Dinghua; Song, Dandan; Gong, Jingming; Yuan, Shuang] Cent China Normal Univ, Coll Chem, Inst Environm Chem, Key Lab Pesticide & Chem Biol,Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Gong, Jingming] C;Cent China Normal Univ, Coll Chem, Inst Environm Chem, Key Lab Pesticide & Chem Biol,Minist Educ, Wuhan 430079, Peoples R China.
关键词：
Mercury (II);Layered titanates;Stripping analysis;Ion exchange;Heavy metal
摘要：
A highly sensitive and selective Hg(II) sensor has been developed by using layered titanate nanosheets (TNs) as an enhanced sensing platform. The TNs with exchangeable sodium cations located in the interlayer are prepared by a facile hydrothermal route. The as-formed sodium titanate nanosheets (Na-TNs) exhibit a porous nanoarchitectured network. The surface structure and electrochemical performance were systematically investigated. Such a nanostructured TNs-based platform, as a typical cation-exchange material, is highly efficient to capture Hg<sup>2+</sup>, which dramatically facilitates the enrichment of Hg <sup>2+</sup> onto their surface and realizes the stripping voltammetric detection of mercury ions with a remarkably improved sensitivity and selectivity. The detection limit was found to be as low as 5 ppt (S/N = 3), much below the guideline value from the World Health Organization (WHO). The interference from other heavy metal ions such as Cd<sup>2+</sup>, Mn <sup>2+</sup>, Ni<sup>2+</sup>, Pb<sup>2+</sup> and Cu<sup>2+</sup> ions associated with mercury analysis could be effectively inhibited. Toward the goal for practical applications, the sensor was further evaluated by monitoring Hg(II) in real mushroom samples. ©2013 Elsevier B.V.
语种：
英文
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The Preparation, Characterization and Application of beta-Cyclodextrin/n-Tetradecylamine Covalently Modified Glassy Carbon Electrode
作者：
Li, Xue;Liang, Han;Song, Xuegang* ;Gong, Jingming
期刊：
Nanoscience and Nanotechnology Letters ,2013年5(6):666-672 ISSN：1941-4900
通讯作者：
Song, Xuegang
作者机构：
[Gong, Jingming; Song, Xuegang; Li, Xue; Liang, Han] Cent China Normal Univ, Minist Educ, Coll Chem, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Song, Xuegang] C;Cent China Normal Univ, Minist Educ, Coll Chem, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
beta-Cyclodextrin (beta-CD);n-Tetradecylamine (TDA);Uric Acid (UA);Differential Pulse Voltammetry (DPV)
摘要：
A β-cyclodextrin (β-CD) modified glassy carbon electrode, based on n-tetradecylamine (TDA) covalently modified glassy carbon electrode (TDA/GCE), was successfully fabricated (labeled as β-CD-TDA/GCE), which provides a novel approach to graft the β-CD on the solid substrate. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) technology was used to characterize the β-CD-TDA/GCE and TDA/GCE, respectively. It was proposed that due to the hydrophobic interaction between β-CD and TDA, the inclusion complex could be formed with β-CD ring strung on the hydrocarbon chain of TDA covalently modified GCE. The supramolecular interaction between β-CD and uric acid (UA) improves the sensitivity and selectivity of the UA detection even in the presence of ascorbic scid (AA). By using the differential pulse voltammetry (DPV), a linear response was obtained in the range of 4.0×10<sup>-6</sup> to 5.0×10<sup>-5</sup> M UA. Copyright ©2013 American Scientific Publishers. All rights reserved.
语种：
英文
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Bifunctional sensor of pentachlorophenol and copper ions based on nanostructured hybrid films of humic acid and exfoliated layered double hydroxide via a facile layer-by-layer assembly
作者：
Yuan, Shuang;Peng, Dinghua;Hu, Xianluo;Gong, Jingming*
期刊：
Analytica Chimica Acta ,2013年785:34-42 ISSN：0003-2670
通讯作者：
Gong, Jingming
作者机构：
[Peng, Dinghua; Gong, Jingming; Yuan, Shuang] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Hu, Xianluo] Huazhong Univ Sci & Technol, Coll Mat Sci & Engn, State Key Lab Mat Proc & Die & Mould Technol, Wuhan 430074, Peoples R China.
通讯机构：
[Gong, Jingming] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Bifunctional sensor;Copper ions;Layer-by-layer assembly;Pentachlorophenol
摘要：
A new, highly sensitive bifunctional electrochemical sensor for the simultaneous determination of pentachlorophenol (PCP) and copper ions (Cu2+) has been developed, where organic-inorganic hybrid ultrathin films were fabricated by alternate assembly of humic acid (HA) and exfoliated Mg-Al-layered double hydroxide (LDH) nanosheets onto ITO substrates via a layer-by-layer (LBL) approach. The multilayer films were then characterized by means of UV-vis spectrometry, scanning electron microscopy (SEM), and atomic force microscope (AFM). These films were found to have a relatively smooth surface with almost equal amounts of HA incorporated in each cycle. Its electrochemical performance was systematically investigated. Our results demonstrate that such a newly designed (LDH/HA)n multilayer films, combining the individual properties of HA (dual recognition ability for organic herbicides and metal ions) together with LDH nanosheets (a rigid inorganic matrix), can be applied to the simultaneous analysis of PCP and Cu(II) without interference from each other. The LBL assembled nanoarchitectures were further investigated by X-ray photoelectron spectroscopy (XPS) and infrared spectroscopy (IR), which provides insight for bifunctional sensing behavior. Under the optimized conditions, the detection limit was found to be as low as 0.4nM PCP, well below the guideline value of PCP in drinking water (3.7nM) set by the United States Environmental Protection Agency (U.S. EPA), and 2.0nM Cu2+, much below the guideline value (2.0mgL-1, ~31.2nM) from the World Health Organization (WHO), respectively. Toward the goal for practical applications, this simple and cost-effective probe was further evaluated by monitoring PCP and Cu(II) in water samples. © 2013 Elsevier B.V.
语种：
英文
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Facile synthesis of zirconia nanoparticles-decorated graphene hybrid nanosheets for an enzymeless methyl parathion sensor
作者：
Gong, Jingming* ;Miao, Xingju;Wan, Huifang;Song, Dandan
期刊：
Sensors and Actuators B-Chemical ,2012年162(1):341-347 ISSN：0925-4005
通讯作者：
Gong, Jingming
作者机构：
[Song, Dandan; Gong, Jingming; Wan, Huifang; Miao, Xingju] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.
通讯机构：
[Gong, Jingming] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.
关键词：
Zirconia nanoparticles;Graphene;One-step co-electrodeposition;Organophosphate pesticide;Enzymeless sensor
摘要：
This paper proposed a facile electrochemical approach to the synthesis of high quality zirconia nanoparticles decorated graphene nanosheets (labeled as ZrO2NPs-GNs) onto a cathodic substrate. This facile one-step co-electrodeposition approach for the construction of GNs based hybrid is environmentally friendly, without involving the chemical reduction of GO, and therefore will not result in further contamination. The electrochemically synthesized ZrO2NPs-GNs composite has been carefully characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM) and various electrochemical techniques. Such a nanostructured composite, combining the advantages of ZrO2NPs (high recognition and enrichment capability for phosphoric moieties) together with GNs (large surface area and high conductivity), is highly efficient to capture organophosphate pesticides (OPs). Combined with the square-wave voltammetry, a highly sensitive enzymeless OPs sensor was fabricated using the prepared ZrO2NPs-GNs composite as solid phase extraction (SPE). The detection limit for methyl parathion (MP) in aqueous solutions was determined to be of 0.6 ng mL−1 (S/N = 3). This work provides a green and facile route for the preparation of GNs-based hybrid, and also offers a new promising protocol for OPs analysis.
语种：
英文
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新型功能化纳米组装体在典型环境污染物的去除和检测中的应用
作者：
龚静鸣;官章琼;袁爽;缪杏菊
作者机构：
[龚静鸣; 官章琼; 袁爽; 缪杏菊] 华中师范大学农药与化学生物学教育部重点实验室,化学学院,武汉市珞瑜路152号, 430079
会议名称：
中国化学会第28届学术年会
会议时间：
2012-4-13
会议地点：
成都
会议主办单位：
中国化学会
会议论文集名称：
中国化学会第28届学术年会论文集
关键词：
纳米组装体;重金属离子;农药残留;电化学检测
摘要：
<正>近年来,人们对纳米材料的研究已逐步深入到纳米组装体系的研究,即以纳米微粒或纳米丝、纳米管、纳米球等为基本单元在一维、.二维和三维空间组装排列成具有新型纳米结构的功能纳米组装体。由于其组
语种：
中文
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New multifunctional porous materials based on inorganic-organic hybrid single-walled carbon nanotubes: Gas storage and high-sensitive detection of pesticides
作者：
Wang, Feng;Zhao, Jinbo;Gong, Jingming;Wen, Lili* ;Zhou, Li;...
期刊：
CHEMISTRY-A EUROPEAN JOURNAL ,2012年18(37):11804-11810 ISSN：0947-6539
通讯作者：
Wen, Lili
作者机构：
[Zhao, Jinbo; Gong, Jingming; Zhou, Li; Li, Dongfeng; Wen, Lili; Wang, Feng] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Li, Dongfeng; Wen, Lili] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Coordinat Chem, Nanjing 210093, Jiangsu, Peoples R China.
通讯机构：
[Wen, Lili] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
gas storage;host-guest systems;nanotubes;pesticides;porous materials
摘要：
Single-walled carbon nanotubes (SWNTs) that are covalently functionalized with benzoic acid (SWNT-PhCOOH) can be integrated with transition-metal ions to form 3D porous inorganic-organic hybrid frameworks (SWNT-Zn). In particular, N2-adsorption analysis shows that the BET surface area increases notably from 645.3 to 1209.9 m2 g-1 for SWNTs and SWNT-Zn, respectively. This remarkable enhancement in the surface area of SWNT-Zn is presumably due to the microporous motifs from benzoates coordinated to intercalated zinc ions between the functionalized SWNTs; this assignment was also corroborated by NLDFT pore-size distributions. In addition, the excess-H2-uptake maximum of SWNT-Zn reaches about 3.1 wt. % (12 bar, 77 K), which is almost three times that of the original SWNTs (1.2 wt. % at 12 bar, 77 K). Owing to its inherent conductivity and pore structure, as well as good dispersibility, SWNT-Zn is an effective candidate as a sensitive electrochemical stripping voltammetric sensor for organophosphate pesticides (OPs): By using solid-phase extraction (SPE) with SWNT-Zn-modified glassy carbon electrode, the detection limit of methyl parathion (MP) is 2.3 ng mL -1. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
语种：
英文
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One-step fabrication of layered double hydroxides/graphene hybrid as solid-phase extraction for stripping voltammetric detection of methyl parathion
作者：
Liang, Han;Miao, Xingju;Gong, Jingming*
期刊：
Electrochemistry Communications ,2012年20(1):149-152 ISSN：1388-2481
通讯作者：
Gong, Jingming
作者机构：
[Gong, Jingming; Liang, Han; Miao, Xingju] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Gong, Jingming] C;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
关键词：
Co-electrodeposition;Graphene;Layered double hydroxides;Methyl parathion;Stripping analysis
摘要：
We developed a green and facile electrochemical approach to synthesize novel Ni/Al layered double hydroxides decorated graphene nanosheets hybrid (labeled as LDHs-GNs) on a cathodic substrate. The as-prepared LDHs-GNs composite is highly efficient to capture organophosphate pesticides (OPs), combining the advantages of LDHs (high enrichment capability for OPs by a selective intercalation) together with GNs (large surface area and high conductivity). It dramatically facilitates the enrichment of OPs onto their surface and realizes the sensitive stripping voltammetric detection of methyl parathion (MP) as a model of OPs. The detection limit for MP in aqueous solutions was determined to be of 0.6 ng mL(-1) (S/N = 3). This work provides a green and facile route for the preparation of GN-based hybrid, and also offers a new promising protocol for OP analysis. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.
语种：
英文
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Adsorption of heavy metal ions by hierarchically structured magnetite-carbonaceous spheres
作者：
Gong, Jingming* ;Wang, Xiaoqing;Shao, Xiulan;Yuan, Shuang;Yang, Chenlin;...
期刊：
Talanta ,2012年101:45-52 ISSN：0039-9140
通讯作者：
Gong, Jingming
作者机构：
[Gong, Jingming; Yuan, Shuang; Shao, Xiulan; Yang, Chenlin; Wang, Xiaoqing] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.;[Hu, Xianluo] Huazhong Univ Sci & Technol, State Key Lab Mat Proc & Die & Mould Technol, Coll Mat Sci & Engn, Wuhan 430074, Peoples R China.
通讯机构：
[Gong, Jingming] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.
关键词：
Heavy metal ions;Separation technology;Magnetite;Carbonaceous composites;Adsorption
摘要：
Magnetically driven separation technology has received considerable attention in recent decade for its great potential application. In this work, hierarchically structured magnetite-carbonaceous microspheres (Fe <inf>3</inf>O<inf>4</inf>-C MSs) have been synthesized for the adsorption of heavy metal ions from aqueous solution. Each sphere contains numerous unique rattle-type structured magnetic particles, realizing the integration of rattle-type building unit into microspheres. The as-prepared composites with high BET surface area, hierarchical as well as mesoporous structures, exhibit an excellent adsorption capacity for heavy metal ions and a convenient separation procedure with the help of an external magnet. It was found that the maximum adsorption capacity of the composite toward Pb<sup>2+</sup> was ∼126 mg g<sup>-1</sup>, displaying a high efficiency for the removal of heavy metal ions. The Freundlich adsorption isotherm was applicable to describe the removal processes. Kinetics of the Pb<sup>2+</sup> removal was found to follow pseudo-second-order rate equation. The as-prepared composite of Fe <inf>3</inf>O<inf>4</inf>-C MSs as well as Pb<sup>2+</sup>-adsorbed composite were carefully examined by scanning electron microscopy (SEM), Zeta potential measurements, Fourier transform infrared spectroscopy (FT-IR), nitrogen sorption measurements, and X-ray photoelectron spectroscopy (XPS). Based on the characterization results, a possible mechanism of Pb<sup>2+</sup> removal with the composite of Fe<inf>3</inf>O<inf>4</inf>-C MSs was proposed. ©2012 Elsevier B.V. All rights reserved.
语种：
英文
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Highly sensitive visible light activated photoelectrochemical biosensing of organophosphate pesticide using biofunctional crossed bismuth oxyiodide flake arrays
作者：
Gong, Jingming* ;Wang, Xiaoqing;Li, Xue;Wang, Kewei
期刊：
Biosensors and Bioelectronics ,2012年38(1):43-49 ISSN：0956-5663
通讯作者：
Gong, Jingming
作者机构：
[Wang, Kewei; Gong, Jingming; Li, Xue; Wang, Xiaoqing] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Gong, Jingming] C;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
关键词：
Acetylcholinesterase;BiOI flake arrays;Biosensor;Organophosphate pesticides;Photoelectrochemical detection
摘要：
A new, highly sensitive and selective biosensor for the photoelectrochemical (PEC) detection of organophosphate pesticides (OPs) has been developed, whereby newly synthesized crossed bismuth oxyiodide (BiOI) nanoflake arrays (BiOINFs) are fabricated as a photoactive electrode via a successive ionic layer adsorption and reaction (SILAR) approach. The smart integration of BiOINFs with biomolecules acetylcholinesterase (AChE) yields a novel AChE-BiOINFs hybrid, constructing a three-dimensional (3D) porous network biosensing platform. The composition and surface structure of the sensor were carefully characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and various electrochemical techniques. Such interlaced network architectures, providing better mass transport and allowing more AChE loading per unit area, as well as the intrinsically strong visible light-harvesting effect from BiOI, greatly facilitate the PEC responses. On the basis of the effect of OPs on the photocurrent of AChE-BiOINFs/ITO, a highly sensitive visible light-activated photoelectrochemical biosensor was developed for biosensing OPs. The conditions for OPs detection were optimized by using methyl parathion (MP) as a model OP compound. Under the optimized experimental conditions, our results show that such a newly designed AChE-BiOINFs/ITO photoactive electrode provides remarkably improved sensitivity and selectivity for the biosensing of OPs. The detection limit was found to be as low as about 0.04ngmL-1 (S/N=3). Toward the goal for practical applications, the resulting sensor was further evaluated by monitoring MP in spiked vegetable samples, showing fine applicability for the detection of MP in real samples. © 2012.
语种：
英文
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流动注射式农药残留的电化学生物传感器研制
作者：
官章琼;缪杏菊;龚静鸣
作者机构：
[官章琼; 缪杏菊; 龚静鸣] 华中师范大学农药与化学生物学教育部重点实验室,化学学院,武汉市珞瑜路152号, 430079
会议名称：
中国化学会第28届学术年会
会议时间：
2012-4-13
会议地点：
成都
会议主办单位：
中国化学会
会议论文集名称：
中国化学会第28届学术年会论文集
关键词：
插层水合物;农药残留;薄层流动电化学检测;生物传感器;酶
摘要：
<正>插层水合物水滑石类化合物是一类典型的插层材料,又称为层状双羟基复合金属氢氧化物(简写为LDHs)。阴离子和极性的有机分子作为典型的客体分子可以插层组装到LDHs的层间,形成功能性超分子插
语种：
中文
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实验教学视频制作与学生实验能力培养
作者：
徐晖;王涟漪;许汉英;龚静鸣;宋丹丹
作者机构：
华中师范大学化学实验教学中心;武汉市第二中学,湖北武汉,430079
会议名称：
第十四届全国高等师范院校化学课程结构与教学改革研讨会暨高等师范院校化学实验教学与实验室建设研讨会
会议时间：
2012-11-23
会议地点：
珠海
会议论文集名称：
第十四届全国高等师范院校化学课程结构与教学改革研讨会暨高等师范院校化学实验教学与实验室建设研讨会论文集
关键词：
高等师范院校;化学实验课程;实验教学视频;实验能力
摘要：
随着研究型实验教学改革的深入,化学实验的教学方法和手段不断更新和发展,各种多媒体技术的应用,优化了实验教学过程,提高了教学效率,增强了教学效果,使化学实验的教学模式有了很大的改变.分析化学实验是一门实践性很强的学科,对于培养学生动手能力,规范学生基础实验操作技能具有重要的意义.为此，精心组织、策划和制作了滴定分析基本操作视频录像,并在教学实践中加以应用,有效地培养了学生的综合实验能力,取得了良好的实验教学效果.
语种：
中文
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Novel nanofibrous composite of chitosan-CaCO3 fabricated by electrolytic biomineralization and its cell biocompatibility
作者：
Zhang, Wei;Liu, Ting;Hu, Xianluo;Gong, Jingming*
期刊：
RSC Advances ,2012年2(2):514-519 ISSN：2046-2069
通讯作者：
Gong, Jingming
作者机构：
[Gong, Jingming; Zhang, Wei; Liu, Ting] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Hu, Xianluo] Huazhong Univ Sci & Technol, Coll Mat Sci & Engn, State Key Lab Mat Proc & Die & Mould Technol, Wuhan 430074, Peoples R China.
通讯机构：
[Gong, Jingming] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
摘要：
A nanofibrous network-like CaCO<inf>3</inf>-chi (CaCO<inf>3</inf>-chi NFs) composite has been successfully fabricated in a simple and controllable approach based on one-step electrodeposition. Results confirm that chitosan can behave as a structure-directing agent during the formation of the CaCO<inf>3</inf>-chi composite coating, playing an important role. The as-formed CaCO<inf>3</inf>-chi NFs were observed to be covered by multiple small nanoparticles (NPs) with an average diameter of ∼25 nm. These uniform NPs were aligned along the surface of NFs, constructing a 3D hierarchically interlaced network. Cytocompatibility of these NFs was evaluated by cell morphology and a MTT assay by culturing MC3T3 cells. The results show that the composite coating of CaCO<inf>3</inf>-chi NFs featuring interconnected pores, loose 3D assembly, large surface area, and high mechanical strength, possess excellent cytocompatibility, greatly facilitating cell adhesion and proliferation. Our research demonstrates that the composite of CaCO<inf>3</inf>-chi NFs can be fabricated in an easy manner, i.e. electrolysis induced biomineralization, and act as a promising scaffold for tissue engineering applications. ©2012 The Royal Society of Chemistry.
语种：
英文
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一步电化学法构筑基于CdS-Chitosan纳米复合膜的新型痕量汞检测荧光传感器
作者：
周婷;袁爽;龚静鸣
作者机构：
[周婷; 袁爽; 龚静鸣] 华中师范大学化学学院农药与有机化学教育部重点实验室
会议名称：
第十一届全国电分析化学会议
会议时间：
2011-05-12
会议地点：
山东聊城
会议论文集名称：
第十一届全国电分析化学会议论文集
关键词：
一步电化学法;CdS-Chitosan纳米复合膜;痕量汞检测荧光传感器
摘要：
<正>以"一步共电沉积法"在电化学体系中制备了具有荧光性质的CdS-Chitosan纳米光学探针,该纳米探针的荧光强度能够被溶液中的汞离子所猝灭。通过对CdS荧光强度信号的跟踪检测,可检测水样中Hg2+的浓度。基于此,建立了一种高选择性的以荧光分析检测汞离子的新方法。结果表明,在最佳实验条件下,此法对样本中的汞离子浓度响应的线性范围为0.1~200μM,
语种：
中文
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Facile fabrication of chitosan-calcium carbonate nanowall arrays and their use as a sensitive non-enzymatic organophosphate pesticide sensor
作者：
Gong, Jingming* ;Zhang, Wei;Liu, Ting;Zhang, Lizhi
期刊：
Nanoscale ,2011年3(8):3123-3131 ISSN：2040-3364
通讯作者：
Gong, Jingming
作者机构：
[Zhang, Lizhi; Gong, Jingming; Zhang, Wei; Liu, Ting] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Gong, Jingming] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
摘要：
Novel nanowall arrays of CaCO<inf>3</inf>-chitosan (CaCO<inf>3</inf>-chi) were deposited onto a cathodic substrate by a facile one-step electrodeposition approach. Results demonstrate that chitosan plays an important role in the formation of nanowall arrays. Freestanding well-aligned CaCO<inf>3</inf>-chi nanowall arrays were observed to be uniformly distributed over the whole substrate with a lateral dimension in the micrometre size and an average pore size of ∼400 nm. The as-formed CaCO<inf>3</inf>-chi nanowall arrays featuring interlaced porous network architecture, large surface area, and open boundaries, are highly efficient in the capture of organophosphate pesticides (OPs). Combined with stripping voltammetry, a highly sensitive non-enzymatic OPs sensor was fabricated using the prepared CaCO<inf>3</inf>-chi nanowall arrays for solid phase extraction (SPE). The detection limit for methyl parathion (MP) in aqueous solutions was determined to be 0.8 ng mL<sup>-1</sup> (S/N = 3). The resulting sensor made of novel CaCO<inf>3</inf>-chi nanowall arrays exhibits good reproducibility and acceptable stability. This work not only provides a facile and effective route for the preparation of CaCO<inf>3</inf>-chi nanowall arrays, but also offers a new promising protocol for OPs analysis. ©2011 The Royal Society of Chemistry.
语种：
英文
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电化学诱导生物矿化合成新颖的壳聚糖-碳酸钙纳米材料及其在组织工程中的应用
作者：
张伟;刘婷;龚静鸣
作者机构：
[张伟; 刘婷; 龚静鸣] 华中师范大学化学学院农药与有机化学教育部重点实验室
会议名称：
第十一届全国电分析化学会议
会议时间：
2011-05-12
会议地点：
山东聊城
会议论文集名称：
第十一届全国电分析化学会议论文集
关键词：
电化学;生物矿化;壳聚糖-碳酸钙;纳米材料;组织工程
摘要：
<正>电化学诱导生物矿化得到壳聚糖-碳酸钙的纳米材料。通过改变实验的条件(如:前驱体的浓度,p H值,沉积电位和电沉积时间等),可以得到形貌可控的二维的城墙网状壳聚糖-碳酸钙纳米阵列和三维的纳米线内交联的网状结构。并分别用扫描电子显微镜,透射电子显微镜,X射线粉末衍射仪和接触角对壳聚糖-碳酸钙的纳米材料进行了表征。然后考察了壳聚糖-碳酸钙纳米材料的生物相容性,将MC3T3-E1细胞在壳聚糖-碳酸钙
语种：
中文
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纳米增效型固相提取剂在典型环境污染物的净化和电化学检测中的应用
作者：
张伟;王晓庆;缪杏菊;龚静鸣
作者机构：
[张伟; 王晓庆; 缪杏菊; 龚静鸣] 华中师范大学农药与化学生物学教育部重点实验室,化学学院,武汉市珞瑜路152;[张伟; 王晓庆; 缪杏菊; 龚静鸣] 号,430079
会议名称：
第十六届全国电化学会议
会议时间：
2011-10-13
会议地点：
重庆
会议主办单位：
中国化学会
会议论文集名称：
第十六届全国电化学会议论文集
语种：
中文
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Efficient Removal of Heavy Metal Ions from Aqueous Systems with the Assembly of Anisotropic Layered Double Hydroxide Nanocrystals@Carbon Nanosphere
作者：
Gong, Jingming* ;Liu, Ting;Wang, Xiaoqing;Hu, Xianluo;Zhang, Lizhi
期刊：
Environmental Science & Technology ,2011年45(14):6181-6187 ISSN：0013-936X
通讯作者：
Gong, Jingming
作者机构：
[Zhang, Lizhi; Gong, Jingming; Liu, Ting; Wang, Xiaoqing] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.;[Hu, Xianluo] Huazhong Univ Sci & Technol, State Key Lab Mat Proc & Die & Mold Technol, Coll Mat Sci & Engn, Wuhan 430074, Peoples R China.
通讯机构：
[Gong, Jingming] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.
摘要：
We report on the efficient removal of heavy metal ions from simulated wastewater with a nanostructured assembly. The nanoassembly was obtained via direct assembling the performed anisotropic layered double hydroxide nanocrystals (LDH-NCs) onto the surface of carbon nanospheres (labeled as LDH-NCs@CNs). It was found that the maximum adsorption capacity of the nanoassembly toward Cu<sup>2+</sup> was ∼19.93 mg g<sup>-1</sup> when the initial Cu<sup>2+</sup> concentration was 10.0 mg L<sup>-1</sup>, displaying a high efficiency for the removal of heavy metal ions. The Freundlich adsorption isotherm was applicable to describe the removal processes. Kinetics of the Cu<sup>2+</sup> removal was found to follow pseudo-second-order rate equation. Furthermore, the as-prepared building unit of the assembly, including LDH-NCs, CNs, and the assembly, as well as Cu<sup>2+</sup>-adsorbed assembly, were carefully examined by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), nitrogen sorption measurements, and X-ray photoelectron spectroscopy (XPS). Based on the characterization results, a possible mechanism of Cu<sup>2+</sup> removal with the assembly of LDH-NCs@CNs was proposed. Comparison experiments show that the adsorption capacity of the resulting LDH-NCs@CNs assembly was much higher than its any building unit alone (CNs or LDH-NCs), exhibiting the deliberation of the assembly on water decontamination. This work provides a very efficient, fast and convenient approach for exploring promising nanoassembly materials for water treatment. ©2011 American Chemical Society.
语种：
英文
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