作者机构：
[Zou Xinsheng; Wang Wen-hui] Huazhong Normal Univ, Sch Foreign Language, Wuhan 430079, Peoples R China.
通讯机构：
[Wang Wen-hui] H;Huazhong Normal Univ, Sch Foreign Language, Wuhan 430079, Peoples R China.
会议名称：
2011 First International Conference on High Performance Structures and Materials Engineering (ICHPSM 2011)
会议时间：
2011-01-01
会议地点：
中国北京
会议主办单位：
[Wang Wen-hui;Zou Xinsheng] Huazhong Normal Univ, Sch Foreign Language, Wuhan 430079, Peoples R China.
会议论文集名称：
Advanced Materials Research
摘要：
College English Curriculum (2004) (CE Curriculum hereafter) is issued by China’s Ministry of Education. As a top-down document, it acts as a guide for colleges and universities nation-wide to formulate a school-based curriculum in the light of their specific circumstances. Compared with the previous counterparts, it is a more balanced and democratic national curriculum. Although the present curriculum is for trial implementation, the course rationale is sound and the curriculum is of greater flexibility.
作者机构：
E-Commerce Research Center of Hubei Province, Central China Normal University, Wuhan, 430079, China;Information Management Department, Central China Normal University, Wuhan, 430079, China;Economic & Management School, Wuhan University,Wuhan,430072, China
会议名称：
International Conference on Business Intelligence and Financial Engineering (BIFE 2011)
会议时间：
2011-01-01
会议地点：
Wuhan, Hubei, China
会议论文集名称：
2011 Fourth International Conference on Business Intelligence and Financial Engineering
摘要：
As an important kind of payment tools, the non-financial third-party e-payment platforms (NTEP), have developed rapidly. It also became the difficulty and blind spot of government supervision. First, we described the development of NTEP in China with typical e-payment tools, and analyzed the urgency and problems of supervision. Then we studied main foreign models for references. Finally, we proposed some suggestions for supervision and promoted the standardized development of NTEP.
期刊：
2011 5th International Conference on Bioinformatics and Biomedical Engineering,2011年:1-4 ISSN：2151-7614
作者机构：
[You, Huihui; Zhao, Jingyun; Li, Lingling; Yuan, Junlin] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Huazhong Normal University, Wuhan, China
会议名称：
International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2011)
会议时间：
2011-01-01
会议地点：
Wuhan, China
会议论文集名称：
2011 5th International Conference on Bioinformatics and Biomedical Engineering. [v.1]
摘要：
In order to study the effects of MnO2 nanoparticles on cells of rats liver and kidney, rats liver and kidney cells were exposed to different concentrations (0, 0.05, 0.50, 5.00, 50.00 μg/mL) of MnO2 nanoparticles at 37℃. An hour later, reactive oxygen species (ROS) levels, glutathione (GSH) concentrations and DNA-protein crosslinks (DPC) degrees in cells were measured to detect the adverse effects. Results showed that compared with the control group, under the 0.5 μg/mL MnO2 nanoparticles exposure, neither the concentrations of GSH reduce nor the levels of ROS increase significantly (p>0.05), but the increase of DPC degrees in cells is significant (p
作者机构：
[Hui Xu; Qianqian Yu; Rosa Morello; Melita Fleischmann; Karl-Siegfried Boos] Laboratory of Bioseparation, Institute of Clinical Chemistry, Medical Center of the University of Munich, Munich, 81377, Germany;[Hui Xu] Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079, China
关键词：
On-line Solid Phase Extraction;HPLC;aldehydes;human plasma;restricted access material
摘要：
A unique site-directed, i.e.in-pore and on-column derivatization method was developed for direct and fully automated analysis of aldehydes in human plasma by combining SPE (Solid Phase Extraction) with HPLC-UV.A Restricted Access Material (RAM; LiChrospher ADS C4 (Merck) was selected as SPE-column packing for on-line sample clean-up and site-directed derivatization.High-molecular-weight interfering constituents of sample matrix (e.g.proteins) are eliminated based on size-exclusion chromatography, while low-molecular-weight target analytes (hexanal and heptanal) have free access to the internal pore surface.These properties allow a site-directed, i.e.in-pore derivatization of the extracted analytes.
作者机构：
[Dai, Shengli] Huazhong Normal Univ, Sch Management, Wuhan 430079, Peoples R China.
会议名称：
International Conference on Intelligent Computation and Industrial Application (ICIA2011)
会议时间：
JUN 18-19, 2011
会议地点：
Hong Kong, PEOPLES R CHINA
会议主办单位：
[Dai, Shengli] Huazhong Normal Univ, Sch Management, Wuhan 430079, Peoples R China.
关键词：
Human Resource Risk;Index System;Risk Assessment
摘要：
Human resources risks affect all aspects of business, they will be significant impact on it's efficiency and performance, the research about risk of human resources is necessary. On the basis of previous studies, this paper analyzes the impact of corporate human resources risk factors, and then build a corporate human resource risk evaluation index system,then measured human resources risks with fuzzy mathematics and G-AHP analysis methods.
作者机构：
School of Management, Wuhan University of Technology, Wuhan, 430070, China;School of Management, Central China Normal University, Wuhan, 430079, China
会议名称：
The 8th International Conference on Innovation and Management(第八届创新与管理国际会议 ICIM 2011)
会议时间：
2011-11-30
会议地点：
日本福冈
会议论文集名称：
The 8th International Conference on Innovation and Management(第八届创新与管理国际会议 ICIM 2011)论文集
关键词：
Flexibility;Marketing expenditure;Budget
摘要：
This article analyzes the traditional methods of marketing expenditure budget systemically, and points out that the traditional methods are all short of opera ability. Then a flexible, dynamic idea, which combines resource guide, competition guide and market guide together, is put forward to investigate the marketing expenditure budget. In addition, the paper discusses the application of flexible budget method and rolling budget method in marketing expenditure budget in details, and builds a flexible operational mechanism to adjust the marketing expenditure.
作者机构：
[Jian Wan; Yanliang Ren; Ke Wei; Yongjian Li] Key Laboratory of Pesticide & Chemical Biology(CCNU), Ministry of Education;Department of Chemistry, Central China Normal University, Wuhan 430079, China;[Xin Xu] State Key Laboratory for Physical Chemistry of Solid Surfaces;College for Chemistry and Chemical Engineering, Center for Theoretical Chemistry, Xiamen University, Xiamen 361005, China;[Jian Wan; Yanliang Ren; Ke Wei; Yongjian Li] Department of Chemistry, Central China Normal University, Wuhan 430079, China
作者：
Sun, L. W.*;Zhao, Y.;Niu, L. P.;Jiang, R.;Song, Y.;...
期刊：
Journal of Physics: Conference Series,2011年276(1) ISSN：1742-6588
通讯作者：
Sun, L. W.
作者机构：
[Feng, K.; Song, Y.; Sun, L. W.; Zhao, Y.; Feng, H.; Jiang, R.] Beihua Univ, Life Sci Res Ctr, Jilin, Peoples R China.;[Niu, L. P.; Qi, C.] Huazhong Normal Univ, Coll Life Sci, Wuhan, Peoples R China.
通讯机构：
[Sun, L. W.] B;Beihua Univ, Life Sci Res Ctr, Jilin, Peoples R China.
会议名称：
3rd International Photonics and OptoElectronics Meetings
会议时间：
NOV 02-05, 2010
会议地点：
Wuhan, PEOPLES R CHINA
会议主办单位：
[Sun, L. W.;Zhao, Y.;Jiang, R.;Song, Y.;Feng, H.;Feng, K.] Beihua Univ, Life Sci Res Ctr, Jilin, Peoples R China.^[Niu, L. P.;Qi, C.] Huazhong Normal Univ, Coll Life Sci, Wuhan, Peoples R China.
会议论文集名称：
Journal of Physics Conference Series
摘要：
Pichia secretive expression system is one of powerful eukaryotic expression systems in genetic engineering, which is especially suitable for industrial utilization. Because of the low concentration of the target protein in initial experiment, the methods and conditions for expression of the target protein should be optimized according to the protein yield repetitively. It is necessary to set up a rapid, simple and convenient analysis method for protein expression levels instead of the generally used method such as ultrafiltration, purification, dialysis, lyophilization and so on. In this paper, acetone precipitation method was chosen to concentrate the recombinant protein firstly after comparing with four different protein precipitation methods systematically, and then the protein was analyzed by SDS-Polyacrylamide Gel Electrophoresis. The recombinant protein was determined with the feature of protein band by the Automated Image Capture and 1-D Analysis Software directly. With this method, the optimized expression conditions of basic fibroblast growth factor secreted from pichia were obtained, which is as the same as using traditional methods. Hence, a convenient tool to determine the optimized conditions for the expression of recombinant proteins in Pichia was established.
